Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G02280 - ( TOC33 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 33) GTP binding / GTPase/ protein binding )

12 Proteins interacs with AT1G02280
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT4G02510

Experimental

pull down

Affinity Capture-Western

affinity technology

FSW = 0.3268

Class A:

plastid

Class B:

unclear

cytosol

Class D:

mitochondrion (p = 0.82)

 TOC159 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 159) TRANSMEMBRANE RECEPTOR
AT1G06950

Experimental

Affinity Capture-Western

affinity technology

FSW = 0.1875

Class A:

plastid

Class D:

plastid (p = 0.78)

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 TIC110 (TRANSLOCON AT THE INNER ENVELOPE MEMBRANE OF CHLOROPLASTS 110)
AT5G54190

Experimental

coimmunoprecipitation

FSW = 0.1778

Class A:

plastid

Class D:

plastid (p = 0.78)

 PORA OXIDOREDUCTASE/ PROTOCHLOROPHYLLIDE REDUCTASE
AT5G55990

Experimental

competition binding

gtpase assay

FSW = 0.0464

Class B:

vacuole

plastid

Class D:

cytosol (p = 0.67)

 CBL2 (CALCINEURIN B-LIKE 2) CALCIUM ION BINDING
AT4G34460

Experimental

biochemical

FSW = 0.0142

Class B:

plastid

plasma membrane

nucleus

golgi

cytosol

 AGB1 (GTP BINDING PROTEIN BETA 1) GTPASE/ NUCLEOTIDE BINDING / PROTEIN BINDING
AT1G35670

Experimental

two hybrid

FSW = 0.0479

Class B:

plastid

plasma membrane

nucleus

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

 ATCDPK2 (CALCIUM-DEPENDENT PROTEIN KINASE 2) CALMODULIN-DEPENDENT PROTEIN KINASE/ KINASE
AT4G09570

Experimental

two hybrid

FSW = 0.0317

Class B:

plastid

nucleus

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

 CPK4 CALMODULIN-DEPENDENT PROTEIN KINASE/ KINASE/ PROTEIN KINASE
AT5G35620

Experimental

FSW = 0.2215

Class B:

plastid

nucleus

cytosol

Class D:

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 LSP1 (LOSS OF SUSCEPTIBILITY TO POTYVIRUS 1) RNA 7-METHYLGUANOSINE CAP BINDING / RNA BINDING / TRANSLATION INITIATION FACTOR
AT5G56280

Experimental

FSW = 0.0667

Class D:

plastid (p = 0.78)

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 CSN6A
AT3G62980

Experimental

filter binding

FSW = 0.0978

Unknown

 TIR1 (TRANSPORT INHIBITOR RESPONSE 1) AUXIN BINDING / PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G35450

Experimental

Reconstituted Complex

in vitro

pull down

FSW = 0.0608

Unknown

 AKR2 (ANKYRIN REPEAT-CONTAINING PROTEIN 2) PROTEIN BINDING
AT5G05000

Predicted

Phylogenetic profile method

Co-expression

FSW = 0.3989

Class C:

plastid

 TOC34 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 34) GTP BINDING

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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