Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G09530 - ( PIF3 (PHYTOCHROME INTERACTING FACTOR 3) DNA binding / protein binding / transcription factor/ transcription regulator )

21 Proteins interacs with AT1G09530
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT2G18790

Experimental

in vitro

in vitro

two hybrid

two hybrid

in vitro

two hybrid

in vitro

in vitro

in vitro

two hybrid

in vitro

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

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interaction detection method

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interaction detection method

two hybrid

Phenotypic Suppression

Affinity Capture-Western

two hybrid

FSW = 0.3125

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

PHYB (PHYTOCHROME B) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT1G09570

Experimental

two hybrid

two hybrid

in vitro

two hybrid

in vitro

in vitro

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

interaction detection method

pull down

two hybrid

Affinity Capture-Western

FSW = 0.2844

Class A:

nucleus

Class B:

cytosol

PHYA (PHYTOCHROME A) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT5G35840

Experimental

FSW = 0.3077

Class A:

nucleus

Class D:

nucleus (p = 0.78)

PHYC (PHYTOCHROME DEFECTIVE C) PROTEIN HISTIDINE KINASE
AT4G15090

Experimental

FSW = 0.1111

Class A:

nucleus

Class D:

nucleus (p = 0.78)

FAR1 (FAR-RED IMPAIRED RESPONSE 1) TRANSCRIPTION ACTIVATOR/ TRANSCRIPTION FACTOR
AT2G01570

Experimental

Reconstituted Complex

two hybrid

in vitro

FSW = 0.0863

Class A:

nucleus

Class D:

nucleus (p = 0.78)

RGA1 (REPRESSOR OF GA1-3 1) PROTEIN BINDING / TRANSCRIPTION FACTOR
AT1G14920

Experimental

two hybrid

FSW = 0.1440

Class A:

nucleus

Class D:

nucleus (p = 0.78)

GAI (GIBBERELLIC ACID INSENSITIVE) TRANSCRIPTION FACTOR
AT5G17490

Experimental

two hybrid

FSW = 0.1600

Class A:

nucleus

Class D:

nucleus (p = 0.78)

RGL3 (RGA-LIKE PROTEIN 3) TRANSCRIPTION FACTOR
AT4G18960

Experimental

FSW = 0.0202

Class A:

nucleus

Class D:

nucleus (p = 0.78)

AG (AGAMOUS) DNA BINDING / TRANSCRIPTION FACTOR
AT1G02340

Experimental

in vitro

Reconstituted Complex

two hybrid

FSW = 0.2449

Class A:

nucleus

Class D:

nucleus (p = 0.78)

HFR1 (LONG HYPOCOTYL IN FAR-RED) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT5G61380

Experimental

two hybrid

two hybrid

two hybrid

two hybrid

FSW = 0.0821

Class A:

nucleus

Class D:

nucleus (p = 0.78)

TOC1 (TIMING OF CAB EXPRESSION 1) TRANSCRIPTION REGULATOR/ TWO-COMPONENT RESPONSE REGULATOR
AT4G16250

Experimental

FSW = 0.2735

Class A:

nucleus

PHYD (PHYTOCHROME DEFECTIVE D) G-PROTEIN COUPLED PHOTORECEPTOR/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT1G66350

Experimental

two hybrid

FSW = 0.1600

Class A:

nucleus

RGL1 (RGA-LIKE 1) TRANSCRIPTION FACTOR
AT2G26300

Experimental

gtpase assay

FSW = 0.0440

Class B:

plasma membrane

nucleus

GP ALPHA 1 (G PROTEIN ALPHA SUBUNIT 1) GTP BINDING / GTPASE/ CHANNEL REGULATOR/ SIGNAL TRANSDUCER
AT5G02810

Experimental

Phenotypic Suppression

FSW = 0.2051

Class B:

nucleus

mitochondrion

Class D:

nucleus (p = 0.78)

PRR7 (PSEUDO-RESPONSE REGULATOR 7) TRANSCRIPTION REGULATOR/ TWO-COMPONENT RESPONSE REGULATOR
AT3G03450

Experimental

two hybrid

FSW = 0.1029

Class D:

nucleus (p = 0.78)

RGL2 (RGA-LIKE 2) TRANSCRIPTION FACTOR
AT2G43010

Experimental

Phenotypic Enhancement

two hybrid

Reconstituted Complex

FSW = 0.5208

Class D:

nucleus (p = 0.78)

PIF4 (PHYTOCHROME INTERACTING FACTOR 4) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT5G20850

Experimental

FSW = 0.0034

Unknown

ATRAD51 ATP BINDING / DNA BINDING / DNA-DEPENDENT ATPASE/ DAMAGED DNA BINDING / NUCLEOSIDE-TRIPHOSPHATASE/ NUCLEOTIDE BINDING / PROTEIN BINDING / SEQUENCE-SPECIFIC DNA BINDING
AT5G61270

Experimental

Phenotypic Enhancement

interaction detection method

FSW = 0.1142

Unknown

PIF7 (PHYTOCHROME-INTERACTING FACTOR7) DNA BINDING / DOUBLE-STRANDED DNA BINDING / TRANSCRIPTION FACTOR
AT4G27630

Experimental

gtpase assay

filter binding

gtpase assay

FSW = 0.2735

Unknown

GTG2 (GPCR-TYPE G PROTEIN 2)
AT1G64990

Experimental

gtpase assay

FSW = 0.2238

Unknown

GTG1 (GPCR-TYPE G PROTEIN 1)
AT3G59060

Experimental

two hybrid

FSW = 0.3054

Unknown

PIL6 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 6) DNA BINDING / TRANSCRIPTION FACTOR

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454