Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G09700 - ( HYL1 (HYPONASTIC LEAVES 1) double-stranded RNA binding / miRNA binding / protein binding )

13 Proteins interacs with AT1G09700
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT2G27100

Experimental

protein complementation assay

two hybrid

Affinity Capture-Western

split-reporter assay

FSW = 0.0933

Class A:

nucleus

Class B:

plastid

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

 SE (SERRATE) DNA BINDING / TRANSCRIPTION FACTOR
AT1G75950

Experimental

pull down

FSW = 0.0176

Class A:

nucleus

Class B:

plasma membrane

cytosol

 SKP1 (S PHASE KINASE-ASSOCIATED PROTEIN 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G03300

Experimental

far western blotting

FSW = 0.4500

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

 DCL2 (DICER-LIKE 2) ATP BINDING / ATP-DEPENDENT HELICASE/ RNA BINDING / DOUBLE-STRANDED RNA BINDING / HELICASE/ NUCLEIC ACID BINDING / RIBONUCLEASE III
AT3G43920

Experimental

far western blotting

FSW = 0.4785

Class A:

nucleus

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

 RIBONUCLEASE III FAMILY PROTEIN
AT3G62800

Experimental

far western blotting

FSW = 0.3025

Class A:

nucleus

Class D:

nucleus (p = 0.78)

 DRB4 (DOUBLE-STRANDED-RNA-BINDING PROTEIN 4) DOUBLE-STRANDED RNA BINDING / PROTEIN BINDING
AT1G01040

Experimental

far western blotting

pull down

protein complementation assay

far western blotting

Affinity Capture-Western

Reconstituted Complex

coimmunoprecipitation

split-reporter assay

FSW = 0.2593

Class A:

nucleus

 DCL1 (DICER-LIKE 1) ATP-DEPENDENT HELICASE/ DOUBLE-STRANDED RNA BINDING / PROTEIN BINDING / RIBONUCLEASE III
AT4G01900

Experimental

FSW = 0.0500

Class B:

plastid

nucleus

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

mitochondrion (p = 0.82)

 GLB1 (GLNB1 HOMOLOG) ACETYLGLUTAMATE KINASE REGULATOR
AT2G28380

Experimental

far western blotting

FSW = 0.2742

Class B:

nucleus

cytosol

Class D:

nucleus (p = 0.78)

mitochondrion (p = 0.82)

 DRB2 (DSRNA-BINDING PROTEIN 2) DOUBLE-STRANDED RNA BINDING
AT1G48410

Experimental

protein complementation assay

split-reporter assay

FSW = 0.2092

Class D:

nucleus (p = 0.78)

 AGO1 (ARGONAUTE 1) ENDORIBONUCLEASE/ MIRNA BINDING / PROTEIN BINDING / SIRNA BINDING
AT5G41070

Experimental

far western blotting

FSW = 0.3741

Unknown

 DRB5 (DSRNA-BINDING PROTEIN 5) DOUBLE-STRANDED RNA BINDING
AT5G42190

Experimental

pull down

FSW = 0.0115

Unknown

 ASK2 (ARABIDOPSIS SKP1-LIKE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G51375Experimental

Affinity Capture-RNA

affinity technology

FSW = 0.2500

Unknown

 ASK2 (ARABIDOPSIS SKP1-LIKE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT1G73687Experimental

Affinity Capture-RNA

affinity technology

FSW = 0.1667

Unknown

 ASK2 (ARABIDOPSIS SKP1-LIKE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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