Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G54250 - ( NRPB8A DNA-directed RNA polymerase )

37 Proteins interacs with AT1G54250
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT3G21350

Experimental

FSW = 0.1389

Unknown

RNA POLYMERASE TRANSCRIPTIONAL REGULATION MEDIATOR-RELATED
AT4G35800

Predicted

two hybrid

Co-crystal Structure

Co-crystal Structure

Affinity Capture-Western

far western blotting

Affinity Capture-MS

Affinity Capture-Western

Co-purification

Affinity Capture-MS

Reconstituted Complex

interaction prediction

Enriched domain pair

FSW = 0.1932

Unknown

NRPB1 (RNA POLYMERASE II LARGE SUBUNIT) DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT4G07950

Predicted

Affinity Capture-MS

FSW = 0.1970

Unknown

DNA-DIRECTED RNA POLYMERASE III FAMILY PROTEIN
AT3G57660

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

Enriched domain pair

FSW = 0.3269

Unknown

NRPA1 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ ZINC ION BINDING
AT3G16980

Predicted

Co-purification

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.3854

Unknown

NRPB9A DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ NUCLEIC ACID BINDING / TRANSCRIPTION REGULATOR/ ZINC ION BINDING
AT5G09920

Predicted

Affinity Capture-MS

FSW = 0.4410

Unknown

NRPB4 DNA-DIRECTED RNA POLYMERASE
AT3G13940

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.2071

Unknown

DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT3G25940

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

FSW = 0.3787

Unknown

TRANSCRIPTION FACTOR S-II (TFIIS) DOMAIN-CONTAINING PROTEIN
AT2G15430

Predicted

Co-purification

Co-purification

Reconstituted Complex

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Co-purification

Reconstituted Complex

Affinity Capture-Western

interaction prediction

FSW = 0.2147

Unknown

NRPB3 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ PROTEIN DIMERIZATION
AT4G08350

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.1928

Unknown

GTA2 (GLOBAL TRANSCRIPTION FACTOR GROUP A2) STRUCTURAL CONSTITUENT OF RIBOSOME / TRANSCRIPTION ELONGATION REGULATOR/ TRANSCRIPTION FACTOR
AT2G34210

Predicted

Affinity Capture-MS

FSW = 0.1894

Unknown

STRUCTURAL CONSTITUENT OF RIBOSOME / TRANSCRIPTION ELONGATION REGULATOR/ TRANSCRIPTION INITIATION FACTOR
AT1G75510

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.3181

Unknown

TRANSCRIPTION INITIATION FACTOR IIF BETA SUBUNIT (TFIIF-BETA) FAMILY PROTEIN
AT5G59180

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Co-purification

interaction prediction

FSW = 0.5630

Unknown

NRPB7 DNA-DIRECTED RNA POLYMERASE/ RNA BINDING
AT3G52090

Predicted

Affinity Capture-MS

FSW = 0.4780

Unknown

NRPB11 DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT4G21710

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

far western blotting

Enriched domain pair

FSW = 0.3335

Unknown

NRPB2 DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT1G61700

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.6545

Unknown

DNA-DIRECTED RNA POLYMERASE II PUTATIVE (RPB10)
AT2G15400

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Co-purification

FSW = 0.4598

Unknown

NRPE3B DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ PROTEIN DIMERIZATION
AT2G29540

Predicted

Affinity Capture-MS

FSW = 0.1667

Unknown

ATRPC14 (RNA POLYMERASE 14 KDA SUBUNIT) DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ PROTEIN DIMERIZATION
AT1G29940Predicted

Affinity Capture-MS

Affinity Capture-MS

Enriched domain pair

FSW = 0.2528

Unknown

NRPA2 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ RIBONUCLEOSIDE BINDING
AT1G06790

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

FSW = 0.1839

Unknown

RNA POLYMERASE RPB7 N-TERMINAL DOMAIN-CONTAINING PROTEIN
AT1G15780

Predicted

two hybrid

FSW = 0.1283

Unknown

UNKNOWN PROTEIN
AT1G60620

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

FSW = 0.2104

Unknown

ATRPAC43 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ PROTEIN DIMERIZATION
AT2G44150

Predicted

Affinity Capture-MS

FSW = 0.1197

Unknown

ASHH3 (HISTONE-LYSINE N-METHYLTRANSFERASE ASHH3) HISTONE-LYSINE N-METHYLTRANSFERASE
AT3G22320

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.5457

Unknown

NRPB5 DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT5G41010

Predicted

Affinity Capture-MS

FSW = 0.5436

Unknown

NRPB12 DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT5G45140

Predicted

Affinity Capture-MS

Affinity Capture-MS

Enriched domain pair

FSW = 0.3141

Unknown

NRPC2 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ RIBONUCLEOSIDE BINDING
AT5G60040

Predicted

two hybrid

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

Enriched domain pair

FSW = 0.2882

Unknown

NRPC1 DNA BINDING / DNA-DIRECTED RNA POLYMERASE/ RIBONUCLEOSIDE BINDING / ZINC ION BINDING
AT3G52590Predicted

interologs mapping

FSW = 0.0299

Unknown

UBQ1 (UBIQUITIN EXTENSION PROTEIN 1) PROTEIN BINDING / STRUCTURAL CONSTITUENT OF RIBOSOME
AT4G05320

Predicted

interologs mapping

FSW = 0.0047

Unknown

UBQ10 (POLYUBIQUITIN 10) PROTEIN BINDING
AT5G42950

Predicted

interologs mapping

FSW = 0.0269

Unknown

GYF DOMAIN-CONTAINING PROTEIN
AT5G51940

Predicted

interologs mapping

Affinity Capture-MS

far western blotting

Co-purification

FSW = 0.6036

Unknown

NRPB6A DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT5G58003

Predicted

Affinity Capture-MS

FSW = 0.3396

Unknown

CPL4 (C-TERMINAL DOMAIN PHOSPHATASE-LIKE 4) PHOSPHOPROTEIN PHOSPHATASE
AT1G11475

Predicted

Affinity Capture-MS

FSW = 0.5975

Unknown

NRPB10 DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT2G04630

Predicted

interologs mapping

FSW = 0.6671

Unknown

NRPB6B DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT4G29600

Predicted

two hybrid

FSW = 0.0224

Unknown

CYTIDINE DEAMINASE PUTATIVE / CYTIDINE AMINOHYDROLASE PUTATIVE
AT5G12410

Predicted

two hybrid

FSW = 0.0293

Unknown

THUMP DOMAIN-CONTAINING PROTEIN
AT3G59600

Predicted

Gene fusion method

Phylogenetic profile method

FSW = 0.1172

Unknown

NRPB8B DNA-DIRECTED RNA POLYMERASE

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454