Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT2G18790 - ( PHYB (PHYTOCHROME B) G-protein coupled photoreceptor/ protein histidine kinase/ red or far-red light photoreceptor/ signal transducer )

29 Proteins interacs with AT2G18790
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G75950

Experimental

FSW = 0.0091

Class A:

nucleus

cytosol

Class B:

plasma membrane

Class D:

cytosol (p = 0.67)

SKP1 (S PHASE KINASE-ASSOCIATED PROTEIN 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT1G09570

Experimental

Phenotypic Enhancement

Phenotypic Enhancement

Phenotypic Suppression

synthetic growth defect

FSW = 0.4949

Class A:

nucleus

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

PHYA (PHYTOCHROME A) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT5G57360

Experimental

two hybrid

two hybrid

Reconstituted Complex

in vitro

FSW = 0.0607

Class A:

nucleus

cytosol

Class D:

nucleus (p = 0.78)

cytosol (p = 0.67)

ZTL (ZEITLUPE) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT2G32950

Experimental

two hybrid

two hybrid

coimmunoprecipitation

FSW = 0.0963

Class A:

nucleus

cytosol

Class D:

nucleus (p = 0.78)

COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT5G63310

Experimental

Reconstituted Complex

Affinity Capture-Western

in vitro

FSW = 0.0352

Class A:

nucleus

Class B:

unclear

plastid

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

NDPK2 (NUCLEOSIDE DIPHOSPHATE KINASE 2) ATP BINDING / NUCLEOSIDE DIPHOSPHATE KINASE/ PROTEIN BINDING
AT5G35840

Experimental

molecular sieving

Affinity Capture-Western

coimmunoprecipitation

affinity technology

FSW = 0.2353

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

cytosol (p = 0.67)

PHYC (PHYTOCHROME DEFECTIVE C) PROTEIN HISTIDINE KINASE
AT5G49230

Experimental

Phenotypic Suppression

FSW = 0.0969

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

HRB1 (HYPERSENSITIVE TO RED AND BLUE) PROTEIN BINDING
AT4G17230

Experimental

synthetic growth defect

FSW = 0.0256

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

SCL13 (SCARECROW-LIKE 13) TRANSCRIPTION FACTOR
AT4G16250

Experimental

affinity technology

Affinity Capture-Western

molecular sieving

Affinity Capture-Western

coimmunoprecipitation

FSW = 0.1328

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

PHYD (PHYTOCHROME DEFECTIVE D) G-PROTEIN COUPLED PHOTORECEPTOR/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT4G18130

Experimental

molecular sieving

Affinity Capture-Western

affinity technology

coimmunoprecipitation

FSW = 0.1558

Class A:

nucleus

Class B:

cytosol

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

PHYE (PHYTOCHROME DEFECTIVE E) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ SIGNAL TRANSDUCER
AT1G09530

Experimental

in vitro

in vitro

two hybrid

two hybrid

in vitro

two hybrid

in vitro

in vitro

in vitro

two hybrid

in vitro

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

pull down

interaction detection method

pull down

pull down

pull down

pull down

interaction detection method

two hybrid

Phenotypic Suppression

Affinity Capture-Western

two hybrid

FSW = 0.3125

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

PIF3 (PHYTOCHROME INTERACTING FACTOR 3) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR/ TRANSCRIPTION REGULATOR
AT1G02340

Experimental

FSW = 0.2408

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

HFR1 (LONG HYPOCOTYL IN FAR-RED) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT1G43700

Experimental

FSW = 0.0250

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

VIP1 (VIRE2-INTERACTING PROTEIN 1) PROTEIN BINDING / TRANSCRIPTION FACTOR
AT2G20180

Experimental

Reconstituted Complex

pull down

coimmunoprecipitation

in vitro

FSW = 0.0611

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

PIL5 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 5) DNA BINDING / PHYTOCHROME BINDING / TRANSCRIPTION FACTOR
AT1G04400

Experimental

Affinity Capture-Western

fluorescence acceptor donor pair

coimmunoprecipitation

affinity technology

FSW = 0.1448

Class A:

nucleus

Class B:

cytosol

Class D:

cytosol (p = 0.67)

CRY2 (CRYPTOCHROME 2) BLUE LIGHT PHOTORECEPTOR/ PROTEIN HOMODIMERIZATION
AT2G02950

Experimental

two hybrid

pull down

Phenotypic Suppression

two hybrid

Reconstituted Complex

in vitro

FSW = 0.0992

Class A:

cytosol

Class B:

nucleus

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

PKS1 (PHYTOCHROME KINASE SUBSTRATE 1) PROTEIN BINDING
AT3G19980

Experimental

in vitro

FSW = 0.0069

Class B:

unclear

nucleus

cytosol

Class D:

cytosol (p = 0.67)

ATFYPP3 (FLOWER-SPECIFIC PHYTOCHROME-ASSOCIATED PROTEIN PHOSPHATASE 3) PROTEIN BINDING / PROTEIN SERINE/THREONINE KINASE/ PROTEIN SERINE/THREONINE PHOSPHATASE
AT1G22280

Experimental

Reconstituted Complex

in vitro

FSW = 0.0552

Class B:

plasma membrane

nucleus

cytosol

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

PROTEIN PHOSPHATASE 2C PUTATIVE / PP2C PUTATIVE
AT5G02810

Experimental

Phenotypic Suppression

FSW = 0.0994

Class B:

nucleus

mitochondrion

cytosol

Class D:

nucleus (p = 0.78)

PRR7 (PSEUDO-RESPONSE REGULATOR 7) TRANSCRIPTION REGULATOR/ TWO-COMPONENT RESPONSE REGULATOR
AT1G04240

Experimental

pull down

Reconstituted Complex

in vitro

FSW = 0.0298

Class D:

plastid (p = 0.78)

nucleus (p = 0.78)

SHY2 (SHORT HYPOCOTYL 2) TRANSCRIPTION FACTOR
AT5G59560

Experimental

Phenotypic Enhancement

FSW = 0.0256

Class D:

nucleus (p = 0.78)

SRR1 (SENSITIVITY TO RED LIGHT REDUCED 1)
AT1G10470

Experimental

two hybrid

affinity technology

Affinity Capture-Western

Reconstituted Complex

coimmunoprecipitation

in vitro

FSW = 0.0357

Class D:

nucleus (p = 0.78)

ARR4 (RESPONSE REGULATOR 4) PROTEIN BINDING / TRANSCRIPTION REGULATOR/ TWO-COMPONENT RESPONSE REGULATOR
AT2G25930

Experimental

two hybrid

Phenotypic Enhancement

Reconstituted Complex

interologs mapping

in vitro

two hybrid

FSW = 0.1587

Class D:

nucleus (p = 0.78)

ELF3 (EARLY FLOWERING 3) PROTEIN C-TERMINUS BINDING / TRANSCRIPTION FACTOR
AT2G43010

Experimental

in vitro

Reconstituted Complex

Reconstituted Complex

Reconstituted Complex

in vitro

coimmunoprecipitation

FSW = 0.3062

Class D:

nucleus (p = 0.78)

PIF4 (PHYTOCHROME INTERACTING FACTOR 4) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT2G42810

Experimental

two hybrid

FSW = 0.0496

Class D:

cytosol (p = 0.67)

PP52 (PROTEIN PHOSPHATASE 52) PHOSPHOPROTEIN PHOSPHATASE/ PROTEIN BINDING / PROTEIN SERINE/THREONINE PHOSPHATASE
AT3G59060

Experimental

in vitro

Reconstituted Complex

Phenotypic Suppression

Phenotypic Suppression

biochemical

Reconstituted Complex

two hybrid

two hybrid

coimmunoprecipitation

FSW = 0.1947

Unknown

PIL6 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 6) DNA BINDING / TRANSCRIPTION FACTOR
AT3G62090

Experimental

coimmunoprecipitation

FSW = 0.0275

Unknown

PIL2 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 2) PROTEIN BINDING / TRANSCRIPTION FACTOR
AT5G61270

Experimental

interaction detection method

Reconstituted Complex

coimmunoprecipitation

in vitro

FSW = 0.0923

Unknown

PIF7 (PHYTOCHROME-INTERACTING FACTOR7) DNA BINDING / DOUBLE-STRANDED DNA BINDING / TRANSCRIPTION FACTOR
AT1G76500

Experimental

Phenotypic Suppression

FSW = 0.1000

Unknown

SOB3 (SUPPRESSOR OF PHYB-4#3) DNA BINDING

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454