Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT2G21410 - ( VHA-A2 (VACUOLAR PROTON ATPASE A2) ATPase )

24 Proteins interacs with AT2G21410
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT4G39080

Predicted

Gene fusion method

Phylogenetic profile method

Co-expression

FSW = 0.1471

Class C:

vacuole

plastid

VHA-A3 (VACUOLAR PROTON ATPASE A3) ATPASE
AT1G76030

Predicted

Affinity Capture-Western

Affinity Capture-Western

co-fractionation

Co-fractionation

Affinity Capture-MS

interaction prediction

FSW = 0.2689

Class C:

vacuole

plastid

VACUOLAR ATP SYNTHASE SUBUNIT B / V-ATPASE B SUBUNIT / VACUOLAR PROTON PUMP B SUBUNIT / V-ATPASE 57 KDA SUBUNIT
AT1G78900

Predicted

two hybrid

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

two hybrid

two hybrid

Affinity Capture-Western

co-fractionation

Co-fractionation

Affinity Capture-MS

interaction prediction

FSW = 0.2885

Class C:

vacuole

plastid

VHA-A (VACUOLAR ATP SYNTHASE SUBUNIT A) ATP BINDING / HYDROGEN ION TRANSPORTING ATP SYNTHASE ROTATIONAL MECHANISM / HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT3G42050

Predicted

Reconstituted Complex

co-fractionation

Co-fractionation

Affinity Capture-Western

Reconstituted Complex

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.4481

Class C:

vacuole

plastid

VACUOLAR ATP SYNTHASE SUBUNIT H FAMILY PROTEIN
AT1G20260

Predicted

Affinity Capture-MS

Affinity Capture-Western

co-fractionation

Co-fractionation

FSW = 0.1111

Class C:

vacuole

plastid

HYDROGEN ION TRANSPORTING ATP SYNTHASE ROTATIONAL MECHANISM / HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT1G12840

Predicted

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.2873

Class C:

vacuole

DET3 (DE-ETIOLATED 3) PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT3G28715

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.0738

Class C:

vacuole

H+-TRANSPORTING TWO-SECTOR ATPASE PUTATIVE
AT3G58730

Predicted

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.2744

Class C:

vacuole

VACUOLAR ATP SYNTHASE SUBUNIT D (VATD) / V-ATPASE D SUBUNIT / VACUOLAR PROTON PUMP D SUBUNIT (VATPD)
AT3G01390

Predicted

Affinity Capture-Western

FSW = 0.3529

Class C:

vacuole

VMA10 (VACUOLAR MEMBRANE ATPASE 10) HYDROGEN ION TRANSPORTING ATP SYNTHASE ROTATIONAL MECHANISM
AT3G28710

Predicted

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-Western

co-fractionation

Co-fractionation

FSW = 0.0712

Class C:

vacuole

H+-TRANSPORTING TWO-SECTOR ATPASE PUTATIVE
AT4G02620

Predicted

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.4808

Class C:

vacuole

VACUOLAR ATPASE SUBUNIT F FAMILY PROTEIN
AT4G23710

Predicted

interaction prediction

FSW = 0.1735

Class C:

vacuole

VAG2 HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES
AT1G64200

Predicted

Affinity Capture-Western

co-fractionation

Co-fractionation

FSW = 0.4201

Class C:

vacuole

VHA-E3 (VACUOLAR H+-ATPASE SUBUNIT E ISOFORM 3) PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT2G45130

Predicted

Affinity Capture-Western

FSW = 0.0920

Class C:

plastid

SPX3 (SPX DOMAIN GENE 3)
AT5G42740

Predicted

Affinity Capture-MS

FSW = 0.0329

Unknown

GLUCOSE-6-PHOSPHATE ISOMERASE CYTOSOLIC (PGIC)
AT5G52640

Predicted

Synthetic Lethality

two hybrid

FSW = 0.0085

Unknown

ATHSP901 (HEAT SHOCK PROTEIN 901) ATP BINDING / UNFOLDED PROTEIN BINDING
AT2G26660

Predicted

Affinity Capture-Western

FSW = 0.0756

Unknown

SPX2 (SPX DOMAIN GENE 2)
AT5G37720

Predicted

Affinity Capture-MS

FSW = 0.0092

Unknown

RNA AND EXPORT FACTOR-BINDING PROTEIN PUTATIVE
AT2G28520

Predicted

Gene neighbors method

Phylogenetic profile method

Co-expression

FSW = 0.1607

Unknown

VHA-A1 (VACUOLAR PROTON ATPASE A 1) ATPASE
AT4G33150

Predicted

Affinity Capture-MS

FSW = 0.0726

Unknown

LYSINE-KETOGLUTARATE REDUCTASE/SACCHAROPINE DEHYDROGENASE BIFUNCTIONAL ENZYME
AT2G40730

Predicted

Affinity Capture-MS

FSW = 0.0368

Unknown

HEAT REPEAT-CONTAINING PROTEIN
AT3G08560

Predicted

Affinity Capture-Western

co-fractionation

Co-fractionation

interaction prediction

FSW = 0.3841

Unknown

VHA-E2 (VACUOLAR H+-ATPASE SUBUNIT E ISOFORM 2) HYDROGEN-EXPORTING ATPASE PHOSPHORYLATIVE MECHANISM / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT5G02530

Predicted

Affinity Capture-MS

FSW = 0.0112

Unknown

RNA AND EXPORT FACTOR-BINDING PROTEIN PUTATIVE
AT4G32840

Predicted

in vitro

in vivo

FSW = 0.0329

Unknown

PFK6 (PHOSPHOFRUCTOKINASE 6) 6-PHOSPHOFRUCTOKINASE

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454