Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT2G26300 - ( GP ALPHA 1 (G PROTEIN ALPHA SUBUNIT 1) GTP binding / GTPase/ channel regulator/ signal transducer )

51 Proteins interacs with AT2G26300
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G48270

Experimental

affinity technology

two hybrid

two hybrid

protein complementation assay

Affinity Capture-Western

Reconstituted Complex

in vitro

FSW = 0.0935

Class A:

plasma membrane

Class B:

vacuole

 GCR1 (G-PROTEIN-COUPLED RECEPTOR 1) G-PROTEIN COUPLED RECEPTOR
AT5G10450

Experimental

gtpase assay

FSW = 0.0027

Class A:

plasma membrane

Class B:

plastid

peroxisome

nucleus

cytosol

Class D:

cytosol (p = 0.67)

 GRF6 (G-BOX REGULATING FACTOR 6) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT4G34460

Experimental

FSW = 0.1059

Class A:

plasma membrane

Class B:

nucleus

golgi

cytosol

 AGB1 (GTP BINDING PROTEIN BETA 1) GTPASE/ NUCLEOTIDE BINDING / PROTEIN BINDING
AT3G22942

Experimental

FSW = 0.0367

Class A:

plasma membrane

Class B:

golgi

 AGG2 (G-PROTEIN GAMMA SUBUNIT 2)
AT3G26090

Experimental

fluorescence acceptor donor pair

gtpase assay

pull down

FSW = 0.0172

Class A:

plasma membrane

Class D:

cytosol (p = 0.67)

 RGS1 (REGULATOR OF G-PROTEIN SIGNALING 1) G-PROTEIN COUPLED RECEPTOR
AT5G63310

Experimental

Reconstituted Complex

in vitro

FSW = 0.0099

Class B:

unclear

plastid

plasma membrane

nucleus

Class D:

cytosol (p = 0.67)

 NDPK2 (NUCLEOSIDE DIPHOSPHATE KINASE 2) ATP BINDING / NUCLEOSIDE DIPHOSPHATE KINASE/ PROTEIN BINDING
AT3G15730

Experimental

pull down

interaction detection method

Reconstituted Complex

in vitro

FSW = 0.0329

Class B:

plastid

plasma membrane

Class D:

cytosol (p = 0.67)

 PLDALPHA1 (PHOSPHOLIPASE D ALPHA 1) PHOSPHOLIPASE D
AT2G20890

Experimental

pull down

two hybrid

Reconstituted Complex

Affinity Capture-Western

fluorescence acceptor donor pair

coimmunoprecipitation

FSW = 0.0186

Class B:

plastid

plasma membrane

 PSB29
AT5G17020

Experimental

gtpase assay

FSW = 0.0193

Class B:

plasma membrane

nucleus

Class D:

cytosol (p = 0.67)

 XPO1A PROTEIN BINDING / PROTEIN TRANSPORTER/ RECEPTOR
AT4G32551

Experimental

FSW = 0.0180

Class B:

plasma membrane

nucleus

 LUG (LEUNIG) PROTEIN BINDING / PROTEIN HETERODIMERIZATION/ TRANSCRIPTION REPRESSOR
AT3G19210

Experimental

FSW = 0.0069

Class B:

plasma membrane

nucleus

 ATRAD54 (ARABIDOPSIS HOMOLOG OF RAD54) ATP BINDING / DNA BINDING / HELICASE/ NUCLEIC ACID BINDING
AT1G09530

Experimental

gtpase assay

FSW = 0.0440

Class B:

plasma membrane

nucleus

 PIF3 (PHYTOCHROME INTERACTING FACTOR 3) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR/ TRANSCRIPTION REGULATOR
AT3G59220

Experimental

pull down

Reconstituted Complex

two hybrid

in vitro

FSW = 0.0196

Class D:

cytosol (p = 0.67)

 PRN (PIRIN) CALMODULIN BINDING
AT4G05420

Experimental

competition binding

FSW = 0.0226

Class D:

cytosol (p = 0.67)

 DDB1A (DAMAGED DNA BINDING PROTEIN 1A) DNA BINDING / PROTEIN BINDING
AT5G24270

Experimental

interaction detection method

FSW = 0.0106

Class D:

cytosol (p = 0.67)

 SOS3 (SALT OVERLY SENSITIVE 3) CALCIUM ION BINDING / CALCIUM-DEPENDENT PROTEIN SERINE/THREONINE PHOSPHATASE
AT1G77140

Experimental

FSW = 0.0156

Class D:

cytosol (p = 0.67)

 VPS45 (VACUOLAR PROTEIN SORTING 45) PROTEIN TRANSPORTER
AT1G69260

Experimental

filter binding

FSW = 0.0200

Unknown

 AFP1 (ABI FIVE BINDING PROTEIN)
AT1G52920

Experimental

two hybrid

coimmunoprecipitation

split-reporter assay

protein complementation assay

Affinity Capture-Western

FSW = 0.0595

Unknown

 GPCR (G PROTEIN COUPLED RECEPTOR) ABSCISIC ACID BINDING / CATALYTIC
AT3G46510

Experimental

gtpase assay

FSW = 0.0196

Unknown

 PUB13 (PLANT U-BOX 13) UBIQUITIN-PROTEIN LIGASE
AT3G59090

Experimental

FSW = 0.0169

Unknown

 EXPRESSED IN 24 PLANT STRUCTURES EXPRESSED DURING 15 GROWTH STAGES BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS TOM1 (TOBAMOVIRUS MULTIPLICATION 1) PROTEIN BINDING (TAIRAT4G217901) HAS 115 BLAST HITS TO 115 PROTEINS IN 19 SPECIES ARCHAE - 0 BACTERIA - 0 METAZOA - 0 FUNGI - 2 PLANTS - 108 VIRUSES - 0 OTHER EUKARYOTES - 5 (SOURCE NCBI BLINK)
AT5G18520

Experimental

FSW = 0.0593

Unknown

 LOCATED IN ENDOMEMBRANE SYSTEM INTEGRAL TO MEMBRANE EXPRESSED IN 24 PLANT STRUCTURES EXPRESSED DURING 14 GROWTH STAGES CONTAINS INTERPRO DOMAIN/S TRANSMEMBRANE RECEPTOR EUKARYOTA (INTERPROIPR009637) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UNKNOWN PROTEIN (TAIRAT3G095701) HAS 385 BLAST HITS TO 381 PROTEINS IN 83 SPECIES ARCHAE - 0 BACTERIA - 2 METAZOA - 213 FUNGI - 14 PLANTS - 131 VIRUSES - 0 OTHER EUKARYOTES - 25 (SOURCE NCBI BLINK)
AT1G70660

Experimental

FSW = 0.0189

Unknown

 MMZ2 (MMS ZWEI HOMOLOGE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G05010

Experimental

FSW = 0.0593

Unknown

 TRANSMEMBRANE PROTEIN PUTATIVE
AT1G57680

Experimental

FSW = 0.0169

Unknown

 FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN LOCATED IN ENDOMEMBRANE SYSTEM EXPRESSED IN 24 PLANT STRUCTURES EXPRESSED DURING 15 GROWTH STAGES CONTAINS INTERPRO DOMAIN/S UNCHARACTERISED CONSERVED PROTEIN UCP031277 (INTERPROIPR016971) HAS 35 BLAST HITS TO 35 PROTEINS IN 10 SPECIES ARCHAE - 0 BACTERIA - 0 METAZOA - 0 FUNGI - 0 PLANTS - 33 VIRUSES - 0 OTHER EUKARYOTES - 2 (SOURCE NCBI BLINK)
AT4G27630

Experimental

two hybrid

Affinity Capture-Western

affinity technology

FSW = 0.0604

Unknown

 GTG2 (GPCR-TYPE G PROTEIN 2)
AT1G64990

Experimental

two hybrid

Affinity Capture-Western

affinity technology

two hybrid

FSW = 0.1039

Unknown

 GTG1 (GPCR-TYPE G PROTEIN 1)
AT2G27820

Experimental

two hybrid

Reconstituted Complex

FSW = 0.0172

Unknown

 PD1 (PREPHENATE DEHYDRATASE 1) AROGENATE DEHYDRATASE/ PREPHENATE DEHYDRATASE
AT3G28710

Predicted

two hybrid

FSW = 0.0037

Class C:

plasma membrane

 H+-TRANSPORTING TWO-SECTOR ATPASE PUTATIVE
AT1G04810

Predicted

Synthetic Rescue

FSW = 0.0164

Class C:

plasma membrane

 26S PROTEASOME REGULATORY SUBUNIT PUTATIVE
AT5G45130

Predicted

two hybrid

FSW = 0.0124

Unknown

 RHA1 (RAB HOMOLOG 1) GTP BINDING
AT4G01370

Predicted

Affinity Capture-Western

Synthetic Rescue

Affinity Capture-Western

Colocalization

Reconstituted Complex

Synthetic Rescue

Phenotypic Suppression

interaction prediction

FSW = 0.0167

Unknown

 ATMPK4 (ARABIDOPSIS THALIANA MAP KINASE 4) MAP KINASE/ KINASE
AT5G35530

Predicted

two hybrid

FSW = 0.0063

Unknown

 40S RIBOSOMAL PROTEIN S3 (RPS3C)
AT5G24090

Predicted

interaction prediction

FSW = 0.0094

Unknown

 ACIDIC ENDOCHITINASE (CHIB1)
AT1G01910

Predicted

Affinity Capture-Western

Reconstituted Complex

biochemical

two hybrid

interaction prediction

FSW = 0.0055

Unknown

 ANION-TRANSPORTING ATPASE PUTATIVE
AT1G60490

Predicted

Reconstituted Complex

Affinity Capture-Western

FSW = 0.0381

Unknown

 ATVPS34 1-PHOSPHATIDYLINOSITOL-3-KINASE/ BINDING / INOSITOL OR PHOSPHATIDYLINOSITOL KINASE/ PHOSPHOTRANSFERASE ALCOHOL GROUP AS ACCEPTOR
AT1G68740

Predicted

interologs mapping

FSW = 0.0818

Unknown

 EXS FAMILY PROTEIN / ERD1/XPR1/SYG1 FAMILY PROTEIN
AT2G15900

Predicted

Affinity Capture-Western

FSW = 0.0494

Unknown

 PHOX (PX) DOMAIN-CONTAINING PROTEIN
AT4G10570

Predicted

Affinity Capture-Western

FSW = 0.0385

Unknown

 UBP9 (UBIQUITIN-SPECIFIC PROTEASE 9) CYSTEINE-TYPE ENDOPEPTIDASE/ UBIQUITIN THIOLESTERASE
AT4G29380

Predicted

Affinity Capture-Western

Reconstituted Complex

FSW = 0.0413

Unknown

 PROTEIN KINASE FAMILY PROTEIN / WD-40 REPEAT FAMILY PROTEIN
AT2G32730

Predicted

Synthetic Rescue

FSW = 0.0119

Unknown

 26S PROTEASOME REGULATORY SUBUNIT PUTATIVE
AT5G40280

Predicted

Synthetic Rescue

FSW = 0.0177

Unknown

 ERA1 (ENHANCED RESPONSE TO ABA 1) FARNESYLTRANSTRANSFERASE/ PROTEIN FARNESYLTRANSFERASE
AT5G55090

Predicted

Synthetic Rescue

FSW = 0.0585

Unknown

 MAPKKK15 ATP BINDING / KINASE/ PROTEIN KINASE/ PROTEIN SERINE/THREONINE KINASE
AT4G36850

Predicted

two hybrid

Reconstituted Complex

FSW = 0.0169

Unknown

 INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN LOCATED IN MEMBRANE CONTAINS INTERPRO DOMAIN/S CYSTINOSIN/ERS1P REPEAT (INTERPROIPR006603) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS PQ-LOOP REPEAT FAMILY PROTEIN / TRANSMEMBRANE FAMILY PROTEIN (TAIRAT2G410501) HAS 599 BLAST HITS TO 441 PROTEINS IN 106 SPECIES ARCHAE - 0 BACTERIA - 0 METAZOA - 197 FUNGI - 311 PLANTS - 38 VIRUSES - 0 OTHER EUKARYOTES - 53 (SOURCE NCBI BLINK)
AT1G32850

Predicted

Affinity Capture-Western

FSW = 0.0818

Unknown

 UBP11 (UBIQUITIN-SPECIFIC PROTEASE 11) CYSTEINE-TYPE ENDOPEPTIDASE/ UBIQUITIN THIOLESTERASE
AT1G10210

Predicted

Affinity Capture-Western

Colocalization

Phenotypic Suppression

Reconstituted Complex

Synthetic Rescue

FSW = 0.0123

Unknown

 ATMPK1 (MITOGEN-ACTIVATED PROTEIN KINASE 1) MAP KINASE/ KINASE
AT1G14040

Predicted

interologs mapping

FSW = 0.0935

Unknown

 LOCATED IN INTEGRAL TO MEMBRANE EXPRESSED IN 21 PLANT STRUCTURES EXPRESSED DURING 12 GROWTH STAGES CONTAINS INTERPRO DOMAIN/S SPX N-TERMINAL (INTERPROIPR004331) EXS C-TERMINAL (INTERPROIPR004342) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UNKNOWN PROTEIN (TAIRAT2G032401) HAS 732 BLAST HITS TO 696 PROTEINS IN 144 SPECIES ARCHAE - 0 BACTERIA - 0 METAZOA - 238 FUNGI - 246 PLANTS - 151 VIRUSES - 0 OTHER EUKARYOTES - 97 (SOURCE NCBI BLINK)
AT5G20350

Predicted

Synthetic Lethality

FSW = 0.0497

Unknown

 TIP1 (TIP GROWTH DEFECTIVE 1) S-ACYLTRANSFERASE/ ACYL BINDING
AT1G31870

Predicted

two hybrid

FSW = 0.0174

Unknown

 UNKNOWN PROTEIN
AT1G30090

Predicted

two hybrid

FSW = 0.0228

Unknown

 KELCH REPEAT-CONTAINING F-BOX FAMILY PROTEIN
AT4G05320

Predicted

interaction prediction

FSW = 0.0035

Unknown

 UBQ10 (POLYUBIQUITIN 10) PROTEIN BINDING
AT4G26050

Predicted

Gene fusion method

FSW = 0.0269

Unknown

 LEUCINE-RICH REPEAT FAMILY PROTEIN

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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