Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT2G32950 - ( COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) protein binding / ubiquitin-protein ligase )

47 Proteins interacs with AT2G32950
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G35160

Experimental

pull down

FSW = 0.0141

Class A:

nucleus

cytosol

Class B:

plasma membrane

cytoskeleton

GF14 PHI (GF14 PROTEIN PHI CHAIN) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT2G18790

Experimental

two hybrid

two hybrid

coimmunoprecipitation

FSW = 0.0963

Class A:

nucleus

cytosol

Class D:

nucleus (p = 0.78)

PHYB (PHYTOCHROME B) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT1G09570

Experimental

Reconstituted Complex

biochemical

Affinity Capture-Western

in vitro

affinity technology

FSW = 0.0952

Class A:

nucleus

cytosol

PHYA (PHYTOCHROME A) G-PROTEIN COUPLED PHOTORECEPTOR/ PROTEIN HISTIDINE KINASE/ RED OR FAR-RED LIGHT PHOTORECEPTOR/ SIGNAL TRANSDUCER
AT4G08920

Experimental

two hybrid

two hybrid

confocal microscopy

two hybrid

Reconstituted Complex

Affinity Capture-Western

coimmunoprecipitation

affinity technology

in vitro

FSW = 0.0667

Class A:

nucleus

cytosol

CRY1 (CRYPTOCHROME 1) ATP BINDING / BLUE LIGHT PHOTORECEPTOR/ PROTEIN HOMODIMERIZATION/ PROTEIN KINASE
AT2G24790

Experimental

two hybrid

FSW = 0.0186

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

COL3 (CONSTANS-LIKE 3) PROTEIN BINDING / TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT5G11260

Experimental

two hybrid

Reconstituted Complex

two hybrid

two hybrid

two hybrid

pull down

biochemical

Reconstituted Complex

in vitro

in vitro

FSW = 0.2759

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

HY5 (ELONGATED HYPOCOTYL 5) DNA BINDING / DOUBLE-STRANDED DNA BINDING / TRANSCRIPTION FACTOR
AT5G15850

Experimental

molecular sieving

FSW = 0.1905

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

COL1 (CONSTANS-LIKE 1) TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT3G54620

Experimental

enzymatic study

FSW = 0.0441

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

BZIP25 (BASIC LEUCINE ZIPPER 25) PROTEIN HETERODIMERIZATION/ TRANSCRIPTION FACTOR
AT3G17609

Experimental

two hybrid

coimmunoprecipitation

FSW = 0.0703

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

HYH (HY5-HOMOLOG) DNA BINDING / TRANSCRIPTION FACTOR
AT1G22770

Experimental

Phenotypic Suppression

protein complementation assay

split-reporter assay

FSW = 0.0586

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

GI (GIGANTEA)
AT5G15840

Experimental

pull down

two hybrid

Reconstituted Complex

fluorescence acceptor donor pair

biochemical

in vitro

FSW = 0.1203

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

CO (CONSTANS) TRANSCRIPTION FACTOR/ TRANSCRIPTION REGULATOR/ ZINC ION BINDING
AT1G02340

Experimental

two hybrid

coimmunoprecipitation

in vitro

two hybrid

Affinity Capture-Western

two hybrid

biochemical

enzymatic study

pull down

pull down

confocal microscopy

Reconstituted Complex

Affinity Capture-Western

coimmunoprecipitation

in vitro

affinity technology

in vitro

FSW = 0.1620

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

HFR1 (LONG HYPOCOTYL IN FAR-RED) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT3G62420

Experimental

FSW = 0.0513

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

ATBZIP53 (BASIC REGION/LEUCINE ZIPPER MOTIF 53) DNA BINDING / PROTEIN HETERODIMERIZATION/ SEQUENCE-SPECIFIC DNA BINDING / TRANSCRIPTION FACTOR
AT2G46340

Experimental

affinity technology

in vitro

co-fractionation

Co-fractionation

affinity technology

affinity technology

Affinity Capture-Western

Affinity Capture-Western

Reconstituted Complex

molecular sieving

confocal microscopy

co-fractionation

Co-fractionation

two hybrid

Reconstituted Complex

Affinity Capture-MS

in vitro

FSW = 0.2279

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

SPA1 (SUPPRESSOR OF PHYA-105 1) PROTEIN BINDING / SIGNAL TRANSDUCER
AT4G18960

Experimental

FSW = 0.0097

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

AG (AGAMOUS) DNA BINDING / TRANSCRIPTION FACTOR
AT4G25560

Experimental

pull down

confocal microscopy

Reconstituted Complex

Affinity Capture-Western

biochemical

in vitro

affinity technology

FSW = 0.0385

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

ATMYB18 (MYB DOMAIN PROTEIN 18) DNA BINDING / TRANSCRIPTION FACTOR
AT2G36890

Experimental

FSW = 0.0800

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

RAX2 (REGULATOR OF AXILLARY MERISTEMS 2) DNA BINDING / TRANSCRIPTION FACTOR
AT4G10180

Experimental

co-fractionation

Co-fractionation

FSW = 0.2078

Class A:

nucleus

Class B:

cytosol

DET1 (DE-ETIOLATED 1) CATALYTIC
AT1G04400

Experimental

Phenotypic Suppression

two hybrid

Affinity Capture-Western

coimmunoprecipitation

FSW = 0.0947

Class A:

nucleus

Class B:

cytosol

CRY2 (CRYPTOCHROME 2) BLUE LIGHT PHOTORECEPTOR/ PROTEIN HOMODIMERIZATION
AT3G07560

Experimental

FSW = 0.0152

Class A:

cytosol

Class B:

peroxisome

nucleus

Class D:

nucleus (p = 0.78)

PEX13 (PEROXIN 13) PROTEIN BINDING
AT5G41790

Experimental

Reconstituted Complex

in vitro

far western blotting

FSW = 0.0060

Class B:

vacuole

plastid

nucleus

cytosol

Class D:

nucleus (p = 0.78)

CIP1 (COP1-INTERACTIVE PROTEIN 1) PROTEIN BINDING
AT4G27430

Experimental

Reconstituted Complex

FSW = 0.0180

Class B:

vacuole

plasma membrane

nucleus

cytosol

Class D:

nucleus (p = 0.78)

CIP7 (COP1-INTERACTING PROTEIN 7) TRANSCRIPTION ACTIVATOR
AT5G24020

Experimental

FSW = 0.0356

Class B:

plastid

nucleus

cytosol

MIND ATPASE/ CALCIUM-DEPENDENT ATPASE/ PROTEIN BINDING / PROTEIN HOMODIMERIZATION
AT1G22280

Experimental

FSW = 0.1008

Class B:

plasma membrane

nucleus

cytosol

Class D:

nucleus (p = 0.78)

PROTEIN PHOSPHATASE 2C PUTATIVE / PP2C PUTATIVE
AT4G21100

Experimental

Reconstituted Complex

Affinity Capture-Western

FSW = 0.1895

Class B:

nucleus

extracellular

cytosol

Class D:

nucleus (p = 0.78)

DDB1B (DAMAGED DNA BINDING PROTEIN 1 B) DAMAGED DNA BINDING / PROTEIN BINDING
AT5G56280

Experimental

molecular sieving

FSW = 0.0993

Class D:

nucleus (p = 0.78)

CSN6A
AT4G05420

Experimental

Reconstituted Complex

FSW = 0.1916

Class D:

nucleus (p = 0.78)

DDB1A (DAMAGED DNA BINDING PROTEIN 1A) DNA BINDING / PROTEIN BINDING
AT4G16110

Experimental

confocal microscopy

FSW = 0.0200

Class D:

nucleus (p = 0.78)

ARR2 (ARABIDOPSIS RESPONSE REGULATOR 2) TRANSCRIPTION FACTOR/ TWO-COMPONENT RESPONSE REGULATOR
AT1G78600

Experimental

enzymatic study

confocal microscopy

FSW = 0.1572

Class D:

nucleus (p = 0.78)

LZF1 (LIGHT-REGULATED ZINC FINGER PROTEIN 1) TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT2G31380

Experimental

fluorescence acceptor donor pair

fluorescence acceptor donor pair

biochemical

two hybrid

in vitro

FSW = 0.1176

Class D:

nucleus (p = 0.78)

STH PROTEIN DOMAIN SPECIFIC BINDING / TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT1G06040

Experimental

two hybrid

FSW = 0.0533

Class D:

nucleus (p = 0.78)

STO (SALT TOLERANCE) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT1G75540

Experimental

confocal microscopy

FSW = 0.1008

Class D:

nucleus (p = 0.78)

STH2 (SALT TOLERANCE HOMOLOG2) TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT5G14250

Experimental

molecular sieving

FSW = 0.0889

Class D:

nucleus (p = 0.78)

COP13 (CONSTITUTIVE PHOTOMORPHOGENIC 13) PROTEIN BINDING
AT5G37190

Experimental

Reconstituted Complex

FSW = 0.0183

Class D:

nucleus (p = 0.78)

CIP4 (COP1-INTERACTING PROTEIN 4) TRANSCRIPTION COFACTOR
AT2G40030

Experimental

FSW = 0.0356

Class D:

nucleus (p = 0.78)

NRPD1B DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT4G02560

Experimental

Phenotypic Suppression

FSW = 0.0399

Class D:

nucleus (p = 0.78)

LD (LUMINIDEPENDENS) TRANSCRIPTION FACTOR
AT2G25930

Experimental

affinity technology

Affinity Capture-Western

two hybrid

biochemical

protein complementation assay

split-reporter assay

in vitro

FSW = 0.1782

Class D:

nucleus (p = 0.78)

ELF3 (EARLY FLOWERING 3) PROTEIN C-TERMINUS BINDING / TRANSCRIPTION FACTOR
AT3G13550

Experimental

Affinity Capture-Western

two hybrid

Affinity Capture-Western

two hybrid

FSW = 0.0713

Unknown

FUS9 (FUSCA 9) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G20050

Experimental

two hybrid

Reconstituted Complex

in vitro

FSW = 0.0056

Unknown

ATTCP-1 ATP BINDING / PROTEIN BINDING / UNFOLDED PROTEIN BINDING
AT5G64920

Experimental

two hybrid

in vitro

Reconstituted Complex

Reconstituted Complex

pull down

pull down

two hybrid

two hybrid

FSW = 0.0392

Unknown

CIP8 (COP1-INTERACTING PROTEIN 8) PROTEIN BINDING / ZINC ION BINDING
AT3G15354

Experimental

affinity technology

Affinity Capture-Western

Affinity Capture-Western

FSW = 0.2467

Unknown

SPA3 (SPA1-RELATED 3) PROTEIN BINDING / SIGNAL TRANSDUCER
AT1G53090

Experimental

affinity technology

Affinity Capture-Western

Affinity Capture-Western

FSW = 0.2842

Unknown

SPA4 (SPA1-RELATED 4) PROTEIN BINDING / SIGNAL TRANSDUCER
AT3G49470

Experimental

FSW = 0.0160

Unknown

NACA2 (NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX SUBUNIT ALPHA-LIKE PROTEIN 2)
AT3G16130

Experimental

FSW = 0.0533

Unknown

ROPGEF13 RHO GUANYL-NUCLEOTIDE EXCHANGE FACTOR
AT1G53720

Experimental

pull down

FSW = 0.0221

Unknown

CYP59 (CYCLOPHILIN 59) RNA BINDING / NUCLEIC ACID BINDING / PEPTIDYL-PROLYL CIS-TRANS ISOMERASE
AT5G46210

Experimental

Affinity Capture-Western

Affinity Capture-Western

affinity technology

FSW = 0.1576

Unknown

CUL4 (CULLIN4) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G11110

Experimental

Affinity Capture-Western

affinity technology

FSW = 0.3567

Unknown

SPA2 (SPA1-RELATED 2) PROTEIN BINDING / SIGNAL TRANSDUCER

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454