Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
Find locus: show subcellular location show Ontology

Export PPI as  



AtPIN will be upgraded. Please send your suggestions here

AT2G46340 - ( SPA1 (SUPPRESSOR OF PHYA-105 1) protein binding / signal transducer )

24 Proteins interacs with AT2G46340
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT2G32950

Experimental

affinity technology

in vitro

co-fractionation

Co-fractionation

affinity technology

affinity technology

Affinity Capture-Western

Affinity Capture-Western

Reconstituted Complex

molecular sieving

confocal microscopy

co-fractionation

Co-fractionation

two hybrid

Reconstituted Complex

Affinity Capture-MS

in vitro

FSW = 0.2279

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT5G15850

Experimental

confocal microscopy

molecular sieving

FSW = 0.2111

Class A:

nucleus

Class D:

nucleus (p = 0.78)

COL1 (CONSTANS-LIKE 1) TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT5G11260

Experimental

FSW = 0.0714

Class A:

nucleus

Class D:

nucleus (p = 0.78)

HY5 (ELONGATED HYPOCOTYL 5) DNA BINDING / DOUBLE-STRANDED DNA BINDING / TRANSCRIPTION FACTOR
AT5G15840

Experimental

Reconstituted Complex

fluorescence acceptor donor pair

in vitro

FSW = 0.1290

Class A:

nucleus

Class D:

nucleus (p = 0.78)

CO (CONSTANS) TRANSCRIPTION FACTOR/ TRANSCRIPTION REGULATOR/ ZINC ION BINDING
AT1G02340

Experimental

two hybrid

Reconstituted Complex

in vitro

coimmunoprecipitation

FSW = 0.0714

Class A:

nucleus

Class D:

nucleus (p = 0.78)

HFR1 (LONG HYPOCOTYL IN FAR-RED) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT5G49760

Experimental

protein array

FSW = 0.0989

Class B:

vacuole

plasma membrane

nucleus

Class D:

nucleus (p = 0.78)

LEUCINE-RICH REPEAT FAMILY PROTEIN / PROTEIN KINASE FAMILY PROTEIN
AT3G56270

Experimental

protein array

FSW = 0.0593

Class B:

vacuole

nucleus

golgi

Class D:

nucleus (p = 0.78)

UNKNOWN PROTEIN
AT4G01900

Experimental

FSW = 0.0296

Class B:

plastid

nucleus

Class D:

nucleus (p = 0.78)

GLB1 (GLNB1 HOMOLOG) ACETYLGLUTAMATE KINASE REGULATOR
AT3G28450

Experimental

protein array

FSW = 0.0228

Class B:

plasma membrane

nucleus

LEUCINE-RICH REPEAT TRANSMEMBRANE PROTEIN KINASE PUTATIVE
AT4G21100

Experimental

Reconstituted Complex

Affinity Capture-Western

FSW = 0.2188

Class B:

nucleus

extracellular

Class D:

nucleus (p = 0.78)

DDB1B (DAMAGED DNA BINDING PROTEIN 1 B) DAMAGED DNA BINDING / PROTEIN BINDING
AT4G05420

Experimental

Reconstituted Complex

FSW = 0.1856

Class D:

nucleus (p = 0.78)

DDB1A (DAMAGED DNA BINDING PROTEIN 1A) DNA BINDING / PROTEIN BINDING
AT3G15354

Experimental

affinity technology

in vitro

Affinity Capture-Western

Reconstituted Complex

confocal microscopy

co-fractionation

Co-fractionation

FSW = 0.3974

Unknown

SPA3 (SPA1-RELATED 3) PROTEIN BINDING / SIGNAL TRANSDUCER
AT1G53090

Experimental

affinity technology

in vitro

Affinity Capture-Western

Reconstituted Complex

confocal microscopy

co-fractionation

Co-fractionation

FSW = 0.4765

Unknown

SPA4 (SPA1-RELATED 4) PROTEIN BINDING / SIGNAL TRANSDUCER
AT5G21170

Experimental

FSW = 0.0126

Unknown

5-AMP-ACTIVATED PROTEIN KINASE BETA-2 SUBUNIT PUTATIVE
AT4G00180

Experimental

pull down

FSW = 0.0988

Unknown

YAB3 (YABBY3) PROTEIN BINDING / TRANSCRIPTION FACTOR
AT1G20950

Experimental

protein array

FSW = 0.0658

Unknown

PYROPHOSPHATE--FRUCTOSE-6-PHOSPHATE 1-PHOSPHOTRANSFERASE-RELATED / PYROPHOSPHATE-DEPENDENT 6-PHOSPHOFRUCTOSE-1-KINASE-RELATED
AT4G23170

Experimental

protein array

FSW = 0.0593

Unknown

EP1 PROTEIN KINASE
AT5G10520

Experimental

protein array

FSW = 0.0423

Unknown

RBK1 (ROP BINDING PROTEIN KINASES 1) ATP BINDING / KINASE/ PROTEIN KINASE/ PROTEIN SERINE/THREONINE KINASE
AT1G74490

Experimental

protein array

FSW = 0.0988

Unknown

PROTEIN KINASE PUTATIVE
AT5G56040

Experimental

protein array

FSW = 0.0918

Unknown

LEUCINE-RICH REPEAT PROTEIN KINASE PUTATIVE
AT4G11110

Experimental

in vitro

Reconstituted Complex

Affinity Capture-Western

co-fractionation

Co-fractionation

affinity technology

co-fractionation

Co-fractionation

FSW = 0.4318

Unknown

SPA2 (SPA1-RELATED 2) PROTEIN BINDING / SIGNAL TRANSDUCER
AT5G22750

Predicted

Gene fusion method

FSW = 0.0450

Class C:

nucleus

RAD5 ATP BINDING / ATP-DEPENDENT HELICASE/ DNA BINDING / HELICASE/ HYDROLASE ACTING ON ACID ANHYDRIDES IN PHOSPHORUS-CONTAINING ANHYDRIDES / NUCLEIC ACID BINDING / PROTEIN BINDING / ZINC ION BINDING
AT1G08590

Predicted

Gene fusion method

FSW = 0.1109

Unknown

CLAVATA1 RECEPTOR KINASE (CLV1)
AT1G63300

Predicted

Gene fusion method

FSW = 0.1078

Unknown

UNKNOWN PROTEIN

Downloads

Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454