Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT3G20740 - ( FIE (FERTILIZATION-INDEPENDENT ENDOSPERM) nucleotide binding / transcription factor/ transcription regulator )

25 Proteins interacs with AT3G20740
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G02580

Experimental

two hybrid

Reconstituted Complex

two hybrid

two hybrid

pull down

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molecular sieving

interaction detection method

Reconstituted Complex

co-fractionation

Co-fractionation

protein complementation assay

Affinity Capture-Western

FSW = 0.2481

Class A:

nucleus

Class B:

cytosol

MEA (MEDEA) SEQUENCE-SPECIFIC DNA BINDING / TRANSCRIPTION FACTOR
AT2G19080

Experimental

FSW = 0.0776

Class B:

unclear

nucleus

mitochondrion

Class D:

cytosol (p = 0.67)

METAXIN-RELATED
AT5G58230

Experimental

molecular sieving

pull down

Affinity Capture-Western

Reconstituted Complex

coimmunoprecipitation

FSW = 0.0672

Class B:

plastid

nucleus

MSI1 (MULTICOPY SUPRESSOR OF IRA1) PROTEIN BINDING
AT2G23380

Experimental

pull down

interaction detection method

two hybrid

Reconstituted Complex

FSW = 0.1731

Class D:

plastid (p = 0.78)

CLF (CURLY LEAF) TRANSCRIPTION FACTOR
AT4G16845

Experimental

coimmunoprecipitation

Affinity Capture-Western

FSW = 0.1504

Unknown

VRN2 (REDUCED VERNALIZATION RESPONSE 2) TRANSCRIPTION FACTOR
AT4G02020

Experimental

interaction detection method

FSW = 0.2924

Unknown

SWN (SWINGER) TRANSCRIPTION FACTOR
AT3G24650

Experimental

FSW = 0.0408

Unknown

ABI3 (ABA INSENSITIVE 3) DNA BINDING / BASAL TRANSCRIPTION REPRESSOR/ TRANSCRIPTION ACTIVATOR/ TRANSCRIPTION FACTOR
AT3G12280

Experimental

pull down

Reconstituted Complex

FSW = 0.0203

Unknown

RBR1 (RETINOBLASTOMA-RELATED 1) TRANSCRIPTION FACTOR BINDING
AT2G22640

Experimental

FSW = 0.0381

Unknown

BRK1 (BRICK1)
ETG5925Experimental

Reconstituted Complex

two hybrid

FSW = 0.1429

Unknown

BRK1 (BRICK1)
AT5G04340

Predicted

Phenotypic Enhancement

Phenotypic Suppression

FSW = 0.0664

Class C:

nucleus

ZAT6 (ZINC FINGER OF ARABIDOPSIS THALIANA 6) NUCLEIC ACID BINDING / TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT4G38130

Predicted

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-Western

co-fractionation

Co-fractionation

Affinity Capture-Western

Affinity Capture-Western

Reconstituted Complex

Co-purification

FSW = 0.0612

Class C:

nucleus

HD1 (HISTONE DEACETYLASE 1) BASAL TRANSCRIPTION REPRESSOR/ HISTONE DEACETYLASE/ PROTEIN BINDING
AT2G01320

Predicted

Phenotypic Suppression

Phenotypic Enhancement

FSW = 0.0593

Unknown

ABC TRANSPORTER FAMILY PROTEIN
AT5G51230

Predicted

Affinity Capture-Western

co-fractionation

Co-fractionation

FSW = 0.1021

Unknown

EMF2 (EMBRYONIC FLOWER 2) DNA BINDING / TRANSCRIPTION FACTOR
AT5G27740

Predicted

two hybrid

FSW = 0.0092

Unknown

EMB2775 (EMBRYO DEFECTIVE 2775) DNA BINDING / NUCLEOSIDE-TRIPHOSPHATASE/ NUCLEOTIDE BINDING
AT3G10070

Predicted

two hybrid

two hybrid

Co-expression

FSW = 0.0136

Unknown

TAF12 (TBP-ASSOCIATED FACTOR 12) DNA BINDING / TRANSCRIPTION INITIATION FACTOR
AT3G61740

Predicted

Phenotypic Suppression

FSW = 0.0200

Unknown

SDG14 (SET DOMAIN PROTEIN 14) DNA BINDING / PROTEIN BINDING / ZINC ION BINDING
AT5G42400

Predicted

Phenotypic Suppression

FSW = 0.1290

Unknown

SDG25 (SET DOMAIN PROTEIN 25)
AT3G23060

Predicted

Phenotypic Suppression

FSW = 0.1078

Unknown

ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN
AT1G30550

Predicted

Reconstituted Complex

two hybrid

FSW = 0.1429

Unknown

FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN EXPRESSED IN 21 PLANT STRUCTURES EXPRESSED DURING 12 GROWTH STAGES BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS WW DOMAIN-CONTAINING PROTEIN (TAIRAT1G452312) HAS 891 BLAST HITS TO 591 PROTEINS IN 275 SPECIES ARCHAE - 68 BACTERIA - 248 METAZOA - 211 FUNGI - 176 PLANTS - 45 VIRUSES - 3 OTHER EUKARYOTES - 140 (SOURCE NCBI BLINK)
AT1G45231

Predicted

Reconstituted Complex

two hybrid

FSW = 0.0635

Unknown

WW DOMAIN-CONTAINING PROTEIN
AT5G10220

Predicted

two hybrid

FSW = 0.0517

Unknown

ANN6 (ANNEXIN ARABIDOPSIS 6) CALCIUM ION BINDING / CALCIUM-DEPENDENT PHOSPHOLIPID BINDING
AT5G47790

Predicted

Affinity Capture-Western

two hybrid

Reconstituted Complex

FSW = 0.0750

Unknown

FORKHEAD-ASSOCIATED DOMAIN-CONTAINING PROTEIN / FHA DOMAIN-CONTAINING PROTEIN
AT5G59160

Predicted

Reconstituted Complex

FSW = 0.0609

Unknown

TOPP2 PROTEIN SERINE/THREONINE PHOSPHATASE
AT4G26800

Predicted

Gene fusion method

FSW = 0.0357

Unknown

PENTATRICOPEPTIDE (PPR) REPEAT-CONTAINING PROTEIN

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454