Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT3G44300 - ( NIT2 (nitrilase 2) indole-3-acetonitrile nitrilase/ indole-3-acetonitrile nitrile hydratase/ nitrilase )

25 Proteins interacs with AT3G44300
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT3G12800

Predicted

Affinity Capture-MS

FSW = 0.0519

Class C:

vacuole

SDRB (SHORT-CHAIN DEHYDROGENASE-REDUCTASE B) BINDING / CATALYTIC/ OXIDOREDUCTASE
AT3G44310

Predicted

Gene neighbors method

Phylogenetic profile method

FSW = 0.0970

Unknown

NIT1 INDOLE-3-ACETONITRILE NITRILASE/ INDOLE-3-ACETONITRILE NITRILE HYDRATASE/ NITRILASE
AT1G26160

Predicted

Affinity Capture-MS

interologs mapping

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

interologs mapping

Synthetic Lethality

Reconstituted Complex

co-fractionation

Co-fractionation

FSW = 0.3295

Unknown

METAL-DEPENDENT PHOSPHOHYDROLASE HD DOMAIN-CONTAINING PROTEIN
AT1G02500

Predicted

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

Reconstituted Complex

Affinity Capture-MS

interologs mapping

Affinity Capture-Western

FSW = 0.1537

Unknown

SAM1 (S-ADENOSYLMETHIONINE SYNTHETASE 1) METHIONINE ADENOSYLTRANSFERASE
AT5G19990

Predicted

Affinity Capture-MS

FSW = 0.0109

Unknown

RPT6A (REGULATORY PARTICLE TRIPLE-A ATPASE 6A) ATPASE
AT2G44510

Predicted

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

two hybrid

Affinity Capture-MS

interologs mapping

interologs mapping

Affinity Capture-Western

FSW = 0.1747

Unknown

P21CIP1-BINDING PROTEIN-RELATED
AT2G40290

Predicted

Affinity Capture-Western

FSW = 0.0235

Unknown

EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1 PUTATIVE / EIF-2A PUTATIVE / EIF-2-ALPHA PUTATIVE
AT5G22300

Predicted

Shared biological function

Phylogenetic profile method

FSW = 0.2370

Unknown

NIT4 (NITRILASE 4) 3-CYANOALANINE HYDRATASE/ CYANOALANINE NITRILASE/ INDOLE-3-ACETONITRILE NITRILASE/ NITRILASE/ NITRILE HYDRATASE
AT3G51895

Predicted

Affinity Capture-Western

Affinity Capture-Western

Reconstituted Complex

biochemical

FSW = 0.1403

Unknown

SULTR31 (SULFATE TRANSPORTER 31) SECONDARY ACTIVE SULFATE TRANSMEMBRANE TRANSPORTER/ SULFATE TRANSMEMBRANE TRANSPORTER/ TRANSPORTER
AT4G39910

Predicted

Affinity Capture-MS

FSW = 0.1536

Unknown

ATUBP3 (ARABIDOPSIS THALIANA UBIQUITIN-SPECIFIC PROTEASE 3) UBIQUITIN-SPECIFIC PROTEASE
AT2G18230

Predicted

Reconstituted Complex

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Synthetic Lethality

Reconstituted Complex

Affinity Capture-Western

Reconstituted Complex

Synthetic Lethality

Co-purification

Affinity Capture-MS

FSW = 0.1322

Unknown

ATPPA2 (ARABIDOPSIS THALIANA PYROPHOSPHORYLASE 2) INORGANIC DIPHOSPHATASE/ PYROPHOSPHATASE
AT3G44320

Predicted

Shared biological function

Gene neighbors method

Phylogenetic profile method

FSW = 0.0736

Unknown

NIT3 (NITRILASE 3) INDOLE-3-ACETONITRILE NITRILASE/ INDOLE-3-ACETONITRILE NITRILE HYDRATASE/ NITRILASE
AT1G50370

Predicted

Affinity Capture-MS

FSW = 0.0672

Unknown

SERINE/THREONINE PROTEIN PHOSPHATASE PUTATIVE
AT1G57660Predicted

interologs mapping

Synthetic Rescue

FSW = 0.0248

Unknown

60S RIBOSOMAL PROTEIN L21 (RPL21E)
AT2G26695

Predicted

interologs mapping

Reconstituted Complex

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

two hybrid

Reconstituted Complex

Affinity Capture-MS

Synthetic Lethality

FSW = 0.3367

Unknown

BINDING / ZINC ION BINDING
AT2G34180

Predicted

Affinity Capture-MS

Affinity Capture-Western

Reconstituted Complex

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-Western

two hybrid

Affinity Capture-MS

two hybrid

two hybrid

Affinity Capture-Western

two hybrid

Affinity Capture-Western

Reconstituted Complex

Synthetic Lethality

FSW = 0.1871

Unknown

CIPK13 (CBL-INTERACTING PROTEIN KINASE 13) ATP BINDING / KINASE/ PROTEIN KINASE/ PROTEIN SERINE/THREONINE KINASE
AT2G41340

Predicted

Affinity Capture-MS

FSW = 0.0388

Unknown

RPB5D (RNA POLYMERASE II FIFTH LARGEST SUBUNIT D) DNA BINDING / DNA-DIRECTED RNA POLYMERASE
AT2G47090

Predicted

Synthetic Lethality

FSW = 0.0229

Unknown

NUCLEIC ACID BINDING / PROTEIN BINDING / ZINC ION BINDING
AT3G16840

Predicted

Affinity Capture-MS

FSW = 0.0205

Unknown

ATP BINDING / ATP-DEPENDENT HELICASE/ HELICASE/ NUCLEIC ACID BINDING
AT3G51880

Predicted

Synthetic Lethality

FSW = 0.0085

Unknown

HMGB1 (HIGH MOBILITY GROUP B 1) DNA BINDING / CHROMATIN BINDING / STRUCTURAL CONSTITUENT OF CHROMATIN / TRANSCRIPTION FACTOR
AT3G54085

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-Western

Reconstituted Complex

FSW = 0.1735

Unknown

FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN LOCATED IN ENDOMEMBRANE SYSTEM CONTAINS INTERPRO DOMAIN/S YOS1-LIKE (INTERPROIPR013880) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UNKNOWN PROTEIN (TAIRAT2G379751) HAS 139 BLAST HITS TO 139 PROTEINS IN 61 SPECIES ARCHAE - 0 BACTERIA - 0 METAZOA - 86 FUNGI - 16 PLANTS - 26 VIRUSES - 0 OTHER EUKARYOTES - 11 (SOURCE NCBI BLINK)
AT4G34880

Predicted

Affinity Capture-Western

Synthetic Lethality

FSW = 0.1214

Unknown

AMIDASE FAMILY PROTEIN
AT5G55190

Predicted

two hybrid

two hybrid

two hybrid

biochemical

biochemical

Reconstituted Complex

Affinity Capture-Western

Synthetic Lethality

Co-purification

FSW = 0.0362

Unknown

RAN3 (RAN GTPASE 3) GTP BINDING / GTPASE/ PROTEIN BINDING
AT5G56740

Predicted

Synthetic Lethality

FSW = 0.0658

Unknown

HAG2 (HISTONE ACETYLTRANSFERASE OF THE GNAT FAMILY 2) H4 HISTONE ACETYLTRANSFERASE/ HISTONE ACETYLTRANSFERASE
AT4G14000

Predicted

two hybrid

two hybrid

FSW = 0.0356

Unknown

UNKNOWN PROTEIN

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Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454