Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT4G02440 - ( EID1 (EMPFINDLICHER IM DUNKELROTEN LICHT 1) ubiquitin-protein ligase )

10 Proteins interacs with AT4G02440
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G75950

Experimental

two hybrid

two hybrid

protein complementation assay

two hybrid

FSW = 0.1372

Class A:

nucleus

Class B:

plasma membrane

cytosol

Class D:

cytosol (p = 0.67)

SKP1 (S PHASE KINASE-ASSOCIATED PROTEIN 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT1G20140

Experimental

protein tri hybrid

two hybrid

fluorescence acceptor donor pair

FSW = 0.2397

Class A:

nucleus

Class D:

cytosol (p = 0.67)

ASK4 (ARABIDOPSIS SKP1-LIKE 4) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G02570

Experimental

protein tri hybrid

fluorescence acceptor donor pair

bioassay

fluorescence acceptor donor pair

FSW = 0.1703

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

ATCUL1 (ARABIDOPSIS THALIANA CULLIN 1) PROTEIN BINDING
AT5G42190

Experimental

two hybrid

two hybrid

fluorescence acceptor donor pair

two hybrid

Reconstituted Complex

in vitro

fluorescence acceptor donor pair

FSW = 0.0916

Unknown

ASK2 (ARABIDOPSIS SKP1-LIKE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G34210

Experimental

protein tri hybrid

two hybrid

fluorescence acceptor donor pair

FSW = 0.2774

Unknown

ASK11 (ARABIDOPSIS SKP1-LIKE 11) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G60010

Experimental

two hybrid

fluorescence acceptor donor pair

FSW = 0.3353

Unknown

ASK13 (ARABIDOPSIS SKP1-LIKE 13) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT2G03170

Experimental

protein tri hybrid

two hybrid

fluorescence acceptor donor pair

FSW = 0.3785

Unknown

ASK14 (ARABIDOPSIS SKP1-LIKE 14) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G21850

Experimental

fluorescence acceptor donor pair

FSW = 0.3871

Unknown

ASK9 (ARABIDOPSIS SKP1-LIKE 9) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT3G25650

Experimental

fluorescence acceptor donor pair

FSW = 0.5246

Unknown

ASK15 (ARABIDOPSIS SKP1-LIKE 15) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT1G10940

Experimental

two hybrid

two hybrid

fluorescence acceptor donor pair

fluorescence acceptor donor pair

Reconstituted Complex

in vitro

fluorescence acceptor donor pair

FSW = 0.0580

Unknown

SNRK24 (SNF1-RELATED PROTEIN KINASE 24) KINASE

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454