Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT4G39400 - ( BRI1 (BRASSINOSTEROID INSENSITIVE 1) kinase/ protein binding / protein heterodimerization/ protein homodimerization/ protein kinase/ protein serine/threonine kinase )

12 Proteins interacs with AT4G39400
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G78300

Experimental

FSW = 0.0505

Class A:

vacuole

plasma membrane

Class B:

peroxisome

nucleus

cytosol

cytoskeleton

Class D:

cytosol (p = 0.67)

GRF2 (GENERAL REGULATORY FACTOR 2) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT4G35230

Experimental

protein kinase assay

FSW = 0.1778

Class A:

vacuole

plasma membrane

Class D:

cytosol (p = 0.67)

BSK1 (BR-SIGNALING KINASE 1) ATP BINDING / BINDING / KINASE/ PROTEIN KINASE/ PROTEIN TYROSINE KINASE
AT1G71830

Experimental

coimmunoprecipitation

in vitro

Affinity Capture-MS

biochemical

biochemical

fluorescence acceptor donor pair

Affinity Capture-Western

coimmunoprecipitation

affinity technology

FSW = 0.1793

Class A:

plasma membrane

Class B:

vacuole

unclear

endoplasmic reticulum

Class D:

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

SERK1 (SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1) KINASE/ TRANSMEMBRANE RECEPTOR PROTEIN SERINE/THREONINE KINASE
AT4G33430

Experimental

two hybrid

in vitro

Affinity Capture-Western

Affinity Capture-Western

biochemical

interaction detection method

Synthetic Rescue

biochemical

Reconstituted Complex

Affinity Capture-Western

fluorescence acceptor donor pair

fluorescence acceptor donor pair

in vitro

in vitro

affinity technology

FSW = 0.2707

Class A:

plasma membrane

Class B:

vacuole

extracellular

Class D:

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

BAK1 (BRI1-ASSOCIATED RECEPTOR KINASE) KINASE/ PROTEIN BINDING / PROTEIN HETERODIMERIZATION/ PROTEIN SERINE/THREONINE KINASE
AT5G42750

Experimental

coimmunoprecipitation

two hybrid

Reconstituted Complex

Affinity Capture-Western

FSW = 0.0896

Class A:

plasma membrane

Class B:

vacuole

cytosol

BKI1 (BRI1 KINASE INHIBITOR 1) PROTEIN HETERODIMERIZATION
AT5G19280

Experimental

Affinity Capture-Western

far western blotting

Co-crystal Structure

Co-crystallization

affinity technology

FSW = 0.2646

Class A:

plasma membrane

Class B:

vacuole

Class D:

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

KAPP (KINASE ASSOCIATED PROTEIN PHOSPHATASE) PHOSPHOPROTEIN PHOSPHATASE/ PROTEIN KINASE BINDING / PROTEIN SERINE/THREONINE PHOSPHATASE
AT5G58220

Experimental

protein kinase assay

two hybrid

co-fractionation

Co-fractionation

FSW = 0.0426

Class B:

vacuole

plasma membrane

peroxisome

Class D:

cytosol (p = 0.67)

TTL (TRANSTHYRETIN-LIKE PROTEIN) STEROID BINDING
AT1G75080

Experimental

biochemical

FSW = 0.1731

Unknown

BZR1 (BRASSINAZOLE-RESISTANT 1) DNA BINDING / TRANSCRIPTION REGULATOR/ TRANSCRIPTION REPRESSOR
AT1G32640

Experimental

FSW = 0.0561

Unknown

MYC2 DNA BINDING / TRANSCRIPTION ACTIVATOR/ TRANSCRIPTION FACTOR
AT2G46280

Experimental

coimmunoprecipitation

Affinity Capture-Western

FSW = 0.0155

Unknown

TRIP-1 (TGF-BETA RECEPTOR INTERACTING PROTEIN 1) NUCLEOTIDE BINDING / PROTEIN BINDING
AT1G55610

Predicted

Phylogenetic profile method

Co-expression

FSW = 0.2395

Class C:

plasma membrane

BRL1 (BRI 1 LIKE) KINASE
AT2G01950

Predicted

Phylogenetic profile method

Co-expression

FSW = 0.0546

Unknown

BRL2 (BRI1-LIKE 2) ATP BINDING / PROTEIN SERINE/THREONINE KINASE/ TRANSMEMBRANE RECEPTOR PROTEIN SERINE/THREONINE KINASE

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Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454