Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT5G05000 - ( TOC34 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 34) GTP binding )

12 Proteins interacs with AT5G05000
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT4G02510

Experimental

gtpase assay

pull down

biochemical

Affinity Capture-Western

affinity technology

FSW = 0.4211

Class A:

unclear

plastid

Class B:

cytosol

Class D:

mitochondrion (p = 0.82)

 TOC159 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 159) TRANSMEMBRANE RECEPTOR
AT2G16640

Experimental

Affinity Capture-Western

affinity technology

FSW = 0.2813

Class A:

unclear

plastid

 TOC132 (MULTIMERIC TRANSLOCON COMPLEX IN THE OUTER ENVELOPE MEMBRANE 132) TRANSMEMBRANE RECEPTOR
AT4G09080

Experimental

FSW = 0.3214

Class A:

plastid

Class B:

unclear

Class D:

cytosol (p = 0.67)

 TOC75-IV
AT3G16620

Experimental

Affinity Capture-Western

affinity technology

FSW = 0.2500

Class A:

plastid

Class B:

unclear

 ATTOC120 GTP BINDING
AT5G35620

Experimental

pull down

FSW = 0.2215

Class B:

unclear

plastid

nucleus

cytosol

Class D:

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 LSP1 (LOSS OF SUSCEPTIBILITY TO POTYVIRUS 1) RNA 7-METHYLGUANOSINE CAP BINDING / RNA BINDING / TRANSLATION INITIATION FACTOR
AT3G50670

Experimental

gtpase assay

FSW = 0.0081

Class B:

unclear

plastid

nucleus

Class D:

mitochondrion (p = 0.82)

 U1-70K (U1 SMALL NUCLEAR RIBONUCLEOPROTEIN-70K) RNA BINDING / NUCLEIC ACID BINDING / NUCLEOTIDE BINDING
AT1G23820

Experimental

biochemical

FSW = 0.0711

Unknown

 SPDS1 (SPERMIDINE SYNTHASE 1) SPERMIDINE SYNTHASE
AT1G45170

Experimental

FSW = 0.3750

Unknown

 UNKNOWN PROTEIN
AT3G62980

Experimental

biochemical

far western blotting

FSW = 0.0978

Unknown

 TIR1 (TRANSPORT INHIBITOR RESPONSE 1) AUXIN BINDING / PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G35450

Experimental

Reconstituted Complex

two hybrid

pull down

in vitro

FSW = 0.0991

Unknown

 AKR2 (ANKYRIN REPEAT-CONTAINING PROTEIN 2) PROTEIN BINDING
AT2G17390

Experimental

two hybrid

FSW = 0.3214

Unknown

 AKR2B (ANKYRIN REPEAT-CONTAINING 2B) PROTEIN BINDING / TRANSCRIPTION REGULATOR
AT1G02280

Predicted

Phylogenetic profile method

Co-expression

FSW = 0.3989

Class C:

plastid

 TOC33 (TRANSLOCON AT THE OUTER ENVELOPE MEMBRANE OF CHLOROPLASTS 33) GTP BINDING / GTPASE/ PROTEIN BINDING

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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