Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT5G22220 - ( E2F1 DNA binding / protein binding / transcription factor )

52 Proteins interacs with AT5G22220
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G78300

Experimental

interaction detection method

FSW = 0.0163

Class A:

nucleus

Class B:

vacuole

plasma membrane

peroxisome

cytosol

cytoskeleton

Class D:

cytosol (p = 0.67)

GRF2 (GENERAL REGULATORY FACTOR 2) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT5G03415

Experimental

protein complementation assay

two hybrid

FSW = 0.1939

Class A:

nucleus

Class D:

nucleus (p = 0.78)

DPB PROTEIN BINDING / PROTEIN HETERODIMERIZATION
AT5G02470

Experimental

pull down

protein complementation assay

two hybrid

coimmunoprecipitation

FSW = 0.2769

Class A:

nucleus

Class D:

nucleus (p = 0.78)

DPA TRANSCRIPTION FACTOR
AT5G23880

Experimental

FSW = 0.0303

Class D:

cytosol (p = 0.67)

CPSF100 (CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 100) DNA BINDING / PROTEIN BINDING
AT3G12280

Experimental

pull down

protein complementation assay

coimmunoprecipitation

FSW = 0.0390

Unknown

RBR1 (RETINOBLASTOMA-RELATED 1) TRANSCRIPTION FACTOR BINDING
AT1G30460

Experimental

FSW = 0.0230

Unknown

CPSF30 RNA BINDING / CALMODULIN BINDING / ENDONUCLEASE/ NUCLEIC ACID BINDING / PROTEIN BINDING
AT2G30360

Experimental

FSW = 0.0535

Unknown

SIP4 (SOS3-INTERACTING PROTEIN 4) KINASE/ PROTEIN KINASE
AT5G11300

Experimental

two hybrid

FSW = 0.1672

Unknown

CYC3B (MITOTIC-LIKE CYCLIN 3B FROM ARABIDOPSIS) CYCLIN-DEPENDENT PROTEIN KINASE REGULATOR
AT2G07698

Predicted

Phenotypic Suppression

FSW = 0.0401

Class C:

nucleus

ATP SYNTHASE ALPHA CHAIN MITOCHONDRIAL PUTATIVE
AT3G48750

Predicted

in vivo

Phenotypic Suppression

Phenotypic Suppression

in vivo

FSW = 0.0225

Class C:

nucleus

CDC2 (CELL DIVISION CONTROL 2) CYCLIN-DEPENDENT PROTEIN KINASE/ KINASE/ PROTEIN BINDING / PROTEIN KINASE
AT5G57015

Predicted

Phenotypic Enhancement

FSW = 0.0066

Class C:

nucleus

CKL12 (CASEIN KINASE I-LIKE 12) ATP BINDING / KINASE/ PROTEIN KINASE/ PROTEIN SERINE/THREONINE KINASE
AT5G53180

Predicted

two hybrid

FSW = 0.0535

Class C:

nucleus

PTB2 (POLYPYRIMIDINE TRACT-BINDING PROTEIN 2) RNA BINDING / NUCLEIC ACID BINDING / NUCLEOTIDE BINDING
AT3G13920

Predicted

Phenotypic Suppression

FSW = 0.0281

Class C:

nucleus

EIF4A1 (EUKARYOTIC TRANSLATION INITIATION FACTOR 4A1) ATP-DEPENDENT HELICASE/ TRANSLATION INITIATION FACTOR
AT4G34430

Predicted

Phenotypic Enhancement

FSW = 0.0113

Class C:

nucleus

CHB3 DNA BINDING / TRANSCRIPTION FACTOR/ ZINC ION BINDING
AT2G27470

Predicted

Phenotypic Enhancement

FSW = 0.0062

Class C:

nucleus

NF-YB11 (NUCLEAR FACTOR Y SUBUNIT B11) TRANSCRIPTION FACTOR
AT5G11510

Predicted

Phenotypic Suppression

Phenotypic Suppression

FSW = 0.1109

Class C:

nucleus

MYB3R-4 (MYB DOMAIN PROTEIN 3R-4) DNA BINDING / TRANSCRIPTION COACTIVATOR/ TRANSCRIPTION FACTOR
AT5G04240

Predicted

in vitro

in vivo

two hybrid

Affinity Capture-MS

FSW = 0.0323

Class C:

nucleus

ELF6 (EARLY FLOWERING 6) TRANSCRIPTION FACTOR
AT5G35530

Predicted

two hybrid

FSW = 0.0062

Class C:

nucleus

40S RIBOSOMAL PROTEIN S3 (RPS3C)
AT5G17020

Predicted

in vitro

in vivo

FSW = 0.0189

Class C:

nucleus

XPO1A PROTEIN BINDING / PROTEIN TRANSPORTER/ RECEPTOR
AT1G36340

Predicted

Phenotypic Enhancement

FSW = 0.0094

Unknown

UBC31 (UBIQUITIN-CONJUGATING ENZYME 31) SMALL CONJUGATING PROTEIN LIGASE/ UBIQUITIN-PROTEIN LIGASE
AT4G38350Predicted

Phenotypic Enhancement

FSW = 0.0364

Unknown

HEDGEHOG RECEPTOR
AT3G15000

Predicted

Phenotypic Enhancement

FSW = 0.0208

Unknown

LOCATED IN MITOCHONDRION EXPRESSED IN 23 PLANT STRUCTURES EXPRESSED DURING 13 GROWTH STAGES BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS PLASTID DEVELOPMENTAL PROTEIN DAG PUTATIVE (TAIRAT3G067902) HAS 50779 BLAST HITS TO 24732 PROTEINS IN 941 SPECIES ARCHAE - 10 BACTERIA - 4862 METAZOA - 25623 FUNGI - 7071 PLANTS - 6957 VIRUSES - 900 OTHER EUKARYOTES - 5356 (SOURCE NCBI BLINK)
AT4G35090

Predicted

two hybrid

FSW = 0.0184

Unknown

CAT2 (CATALASE 2) CATALASE
AT2G27290

Predicted

two hybrid

FSW = 0.0958

Unknown

UNKNOWN PROTEIN
AT3G55200Predicted

two hybrid

FSW = 0.0332

Unknown

SPLICING FACTOR PUTATIVE
AT3G52150

Predicted

two hybrid

FSW = 0.0073

Unknown

RNA RECOGNITION MOTIF (RRM)-CONTAINING PROTEIN
AT4G38740

Predicted

two hybrid

FSW = 0.0201

Unknown

ROC1 (ROTAMASE CYP 1) PEPTIDYL-PROLYL CIS-TRANS ISOMERASE
AT5G13300

Predicted

two hybrid

FSW = 0.0312

Unknown

SFC (SCARFACE) ARF GTPASE ACTIVATOR/ PHOSPHOINOSITIDE BINDING
AT5G15680

Predicted

two hybrid

FSW = 0.0321

Unknown

BINDING
AT5G46630

Predicted

two hybrid

FSW = 0.0139

Unknown

CLATHRIN ADAPTOR COMPLEXES MEDIUM SUBUNIT FAMILY PROTEIN
AT4G31180

Predicted

Phenotypic Suppression

FSW = 0.0247

Unknown

ASPARTYL-TRNA SYNTHETASE PUTATIVE / ASPARTATE--TRNA LIGASE PUTATIVE
AT5G28060

Predicted

two hybrid

FSW = 0.0062

Unknown

40S RIBOSOMAL PROTEIN S24 (RPS24B)
AT5G12020

Predicted

two hybrid

FSW = 0.0153

Unknown

HSP176II (176 KDA CLASS II HEAT SHOCK PROTEIN)
AT2G26210

Predicted

Phenotypic Enhancement

FSW = 0.0247

Unknown

ANKYRIN REPEAT FAMILY PROTEIN
AT4G36080Predicted

Phenotypic Enhancement

FSW = 0.0179

Unknown

FAT DOMAIN-CONTAINING PROTEIN / PHOSPHATIDYLINOSITOL 3- AND 4-KINASE FAMILY PROTEIN
AT5G09740

Predicted

Phenotypic Enhancement

FSW = 0.0117

Unknown

HAM2 (HISTONE ACETYLTRANSFERASE OF THE MYST FAMILY 2) H3/H4 HISTONE ACETYLTRANSFERASE/ HISTONE ACETYLTRANSFERASE/ NUCLEIC ACID BINDING / ZINC ION BINDING
AT5G62310

Predicted

Phenotypic Enhancement

FSW = 0.0172

Unknown

IRE (INCOMPLETE ROOT HAIR ELONGATION) KINASE/ PROTEIN SERINE/THREONINE KINASE
AT1G16590

Predicted

two hybrid

FSW = 0.0663

Unknown

REV7 DNA BINDING
AT1G68200

Predicted

two hybrid

FSW = 0.0108

Unknown

ZINC FINGER (CCCH-TYPE) FAMILY PROTEIN
AT2G44420

Predicted

two hybrid

FSW = 0.0561

Unknown

PROTEIN N-TERMINAL ASPARAGINE AMIDOHYDROLASE FAMILY PROTEIN
AT2G46280

Predicted

two hybrid

FSW = 0.0042

Unknown

TRIP-1 (TGF-BETA RECEPTOR INTERACTING PROTEIN 1) NUCLEOTIDE BINDING / PROTEIN BINDING
AT3G57490

Predicted

two hybrid

FSW = 0.0153

Unknown

40S RIBOSOMAL PROTEIN S2 (RPS2D)
AT4G11330

Predicted

Phenotypic Enhancement

FSW = 0.0242

Unknown

ATMPK5 (MAP KINASE 5) MAP KINASE/ KINASE
AT4G29020

Predicted

two hybrid

FSW = 0.0488

Unknown

GLYCINE-RICH PROTEIN
AT5G16040

Predicted

two hybrid

FSW = 0.0153

Unknown

REGULATOR OF CHROMOSOME CONDENSATION (RCC1) FAMILY PROTEIN
AT5G55130

Predicted

two hybrid

FSW = 0.0165

Unknown

CNX5 (CO-FACTOR FOR NITRATE REDUCTASE AND XANTHINE DEHYDROGENASE 5) MO-MOLYBDOPTERIN COFACTOR SULFURASE
AT5G67320

Predicted

Phenotypic Enhancement

FSW = 0.0321

Unknown

HOS15 (HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15)
AT5G45190

Predicted

two hybrid

FSW = 0.1266

Unknown

CYCLIN FAMILY PROTEIN
AT5G56580

Predicted

two hybrid

FSW = 0.0281

Unknown

MKK6 (MAP KINASE KINASE 6) MAP KINASE KINASE/ KINASE
AT5G67540

Predicted

two hybrid

FSW = 0.0045

Unknown

GLYCOSYL HYDROLASE FAMILY PROTEIN 43
AT3G54180

Predicted

in vivo

in vivo

FSW = 0.0879

Unknown

CDKB11 (CYCLIN-DEPENDENT KINASE B11) CYCLIN-DEPENDENT PROTEIN KINASE/ KINASE/ PROTEIN BINDING
AT3G59060

Predicted

in vitro

in vivo

two hybrid

FSW = 0.0264

Unknown

PIL6 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 6) DNA BINDING / TRANSCRIPTION FACTOR

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454