Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT5G56290 - ( PEX5 (PEROXIN 5) peroxisome matrix targeting signal-1 binding / protein binding )

55 Proteins interacs with AT5G56290
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G20630

Experimental

two hybrid

FSW = 0.0305

Class A:

peroxisome

Class B:

plastid

mitochondrion

Class D:

plastid (p = 0.78)

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 CAT1 (CATALASE 1) CATALASE
AT5G62810

Experimental

two hybrid

FSW = 0.1232

Class A:

peroxisome

Class B:

plasma membrane

Class D:

cytosol (p = 0.67)

 PEX14 PROTEIN BINDING / PROTEIN TRANSPORTER
AT5G27600

Experimental

two hybrid

in vitro

coimmunoprecipitation

Reconstituted Complex

FSW = 0.0162

Class A:

peroxisome

Class D:

plastid (p = 0.78)

cytosol (p = 0.67)

 LACS7 (LONG-CHAIN ACYL-COA SYNTHETASE 7) LONG-CHAIN-FATTY-ACID-COA LIGASE/ PROTEIN BINDING
AT1G29260

Experimental

coimmunoprecipitation

FSW = 0.0726

Class A:

peroxisome

Class D:

mitochondrion (p = 0.82)

cytosol (p = 0.67)

 PEX7 PEROXISOME MATRIX TARGETING SIGNAL-2 BINDING / PROTEIN BINDING
AT5G43280

Predicted

two hybrid

two hybrid

FSW = 0.0394

Class C:

peroxisome

 ATDCI1 (DELTA(35)DELTA(24)-DIENOYL-COA ISOMERASE 1) DELTA35-DELTA24-DIENOYL-COA ISOMERASE/ ENOYL-COA HYDRATASE
AT2G26350

Predicted

in vivo

in vitro

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-Western

Co-expression

FSW = 0.3010

Class C:

peroxisome

 PEX10 PROTEIN BINDING / ZINC ION BINDING
AT3G07560

Predicted

two hybrid

in vitro

in vitro

two hybrid

FSW = 0.0929

Class C:

peroxisome

 PEX13 (PEROXIN 13) PROTEIN BINDING
AT1G79810

Predicted

in vivo

two hybrid

Affinity Capture-MS

FSW = 0.1639

Class C:

peroxisome

 TED3 (REVERSAL OF THE DET PHENOTYPE 3) PROTEIN BINDING / ZINC ION BINDING
AT3G04460

Predicted

two hybrid

two hybrid

in vivo

in vivo

in vitro

in vitro

Co-purification

Affinity Capture-Western

Affinity Capture-Western

Affinity Capture-Western

FSW = 0.1377

Class C:

peroxisome

 PEX12 PROTEIN BINDING / ZINC ION BINDING
AT1G03000

Predicted

co-fractionation

Co-fractionation

FSW = 0.1548

Class C:

peroxisome

 PEX6 (PEROXIN 6) ATP BINDING / ATPASE/ NUCLEOSIDE-TRIPHOSPHATASE/ NUCLEOTIDE BINDING
AT5G08470

Predicted

co-fractionation

Co-fractionation

co-fractionation

Co-fractionation

FSW = 0.1366

Class C:

peroxisome

 PEX1 (PEROXISOME 1) ATP BINDING / ATPASE/ BINDING / NUCLEOSIDE-TRIPHOSPHATASE/ NUCLEOTIDE BINDING / PROTEIN BINDING
AT5G17550

Predicted

two hybrid

FSW = 0.1143

Class C:

peroxisome

 PEX19-2
AT3G03490

Predicted

two hybrid

interaction prediction

FSW = 0.0778

Class C:

peroxisome

 PEX19-1 (PEROXIN 19-1)
AT5G42890

Predicted

two hybrid

two hybrid

two hybrid

FSW = 0.0610

Class C:

peroxisome

 SCP2 (STEROL CARRIER PROTEIN 2) OXIDOREDUCTASE/ STEROL CARRIER
AT2G26230

Predicted

two hybrid

two hybrid

FSW = 0.0552

Class C:

peroxisome

 URICASE / URATE OXIDASE / NODULIN 35 PUTATIVE
AT5G65110

Predicted

two hybrid

Shared biological function

Co-expression

FSW = 0.0485

Class C:

peroxisome

 ACX2 (ACYL-COA OXIDASE 2) ACYL-COA OXIDASE
AT2G35690

Predicted

Reconstituted Complex

two hybrid

FSW = 0.0261

Class C:

peroxisome

 ACX5 (ACYL-COA OXIDASE 5) FAD BINDING / ACYL-COA DEHYDROGENASE/ ACYL-COA OXIDASE/ ELECTRON CARRIER/ OXIDOREDUCTASE/ OXIDOREDUCTASE ACTING ON THE CH-CH GROUP OF DONORS
AT4G16760

Predicted

two hybrid

Reconstituted Complex

interaction prediction

FSW = 0.0566

Class C:

peroxisome

 ACX1 (ACYL-COA OXIDASE 1) ACYL-COA OXIDASE
AT5G66030

Predicted

two hybrid

FSW = 0.0177

Unknown

 ATGRIP PROTEIN BINDING
AT4G16520

Predicted

two hybrid

FSW = 0.0203

Unknown

 ATG8F (AUTOPHAGY 8F) MICROTUBULE BINDING
AT2G05630

Predicted

two hybrid

two hybrid

FSW = 0.0512

Unknown

 AUTOPHAGY 8D (APG8D)
AT3G62870

Predicted

Affinity Capture-MS

synthetic growth defect

FSW = 0.0054

Unknown

 60S RIBOSOMAL PROTEIN L7A (RPL7AB)
AT1G07890

Predicted

Affinity Capture-MS

FSW = 0.0102

Unknown

 APX1 (ASCORBATE PEROXIDASE 1) L-ASCORBATE PEROXIDASE
AT3G48190

Predicted

in vivo

two hybrid

FSW = 0.0115

Unknown

 ATM (ATAXIA-TELANGIECTASIA MUTATED) 1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE
AT5G52640

Predicted

synthetic growth defect

FSW = 0.0100

Unknown

 ATHSP901 (HEAT SHOCK PROTEIN 901) ATP BINDING / UNFOLDED PROTEIN BINDING
AT1G43670

Predicted

co-fractionation

Co-fractionation

FSW = 0.0138

Unknown

 FRUCTOSE-16-BISPHOSPHATASE PUTATIVE / D-FRUCTOSE-16-BISPHOSPHATE 1-PHOSPHOHYDROLASE PUTATIVE / FBPASE PUTATIVE
AT1G50360

Predicted

Affinity Capture-MS

FSW = 0.0436

Unknown

 VIIIA MOTOR
AT3G61670

Predicted

two hybrid

FSW = 0.0690

Unknown

 UNKNOWN PROTEIN
AT1G24310

Predicted

two hybrid

two hybrid

FSW = 0.0349

Unknown

 UNKNOWN PROTEIN
AT2G36170Predicted

two hybrid

FSW = 0.0108

Unknown

 UBIQUITIN EXTENSION PROTEIN 2 (UBQ2) / 60S RIBOSOMAL PROTEIN L40 (RPL40A)
AT3G46560

Predicted

two hybrid

two hybrid

FSW = 0.0345

Unknown

 TIM9 P-P-BOND-HYDROLYSIS-DRIVEN PROTEIN TRANSMEMBRANE TRANSPORTER
AT3G12280

Predicted

Phenotypic Enhancement

FSW = 0.0021

Unknown

 RBR1 (RETINOBLASTOMA-RELATED 1) TRANSCRIPTION FACTOR BINDING
AT3G52590Predicted

two hybrid

FSW = 0.0315

Unknown

 UBQ1 (UBIQUITIN EXTENSION PROTEIN 1) PROTEIN BINDING / STRUCTURAL CONSTITUENT OF RIBOSOME
AT4G17760

Predicted

two hybrid

FSW = 0.0099

Unknown

 DAMAGED DNA BINDING / EXODEOXYRIBONUCLEASE III
AT5G48690

Predicted

two hybrid

FSW = 0.0345

Unknown

 FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN LOCATED IN CELLULAR_COMPONENT UNKNOWN CONTAINS INTERPRO DOMAIN/S UBIQUITIN-ASSOCIATED/TRANSLATION ELONGATION FACTOR EF1B N-TERMINAL (INTERPROIPR000449) UBIQUITIN-ASSOCIATED/TRANSLATION ELONGATION FACTOR EF1B N-TERMINAL EUKARYOTE (INTERPROIPR015940) PUG (INTERPROIPR006567) UBA-LIKE (INTERPROIPR009060) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UBIQUITIN-ASSOCIATED (UBA)/TS-N DOMAIN-CONTAINING PROTEIN (TAIRAT1G048501) HAS 6741 BLAST HITS TO 4773 PROTEINS IN 362 SPECIES ARCHAE - 9 BACTERIA - 355 METAZOA - 2762 FUNGI - 868 PLANTS - 184 VIRUSES - 19 OTHER EUKARYOTES - 2544 (SOURCE NCBI BLINK)
AT1G62940

Predicted

two hybrid

FSW = 0.0381

Unknown

 ACOS5 (ACYL-COA SYNTHETASE 5) 4-COUMARATE-COA LIGASE/ LONG-CHAIN-FATTY-ACID-COA LIGASE/ MEDIUM-CHAIN-FATTY-ACID-COA LIGASE
AT3G21240

Predicted

two hybrid

two hybrid

FSW = 0.0339

Unknown

 4CL2 (4-COUMARATECOA LIGASE 2) 4-COUMARATE-COA LIGASE
AT3G51000

Predicted

two hybrid

two hybrid

FSW = 0.0276

Unknown

 EPOXIDE HYDROLASE PUTATIVE
AT3G63095

Predicted

two hybrid

FSW = 0.0690

Unknown

 ENCODES A PROTEASE INHIBITOR/SEED STORAGE/LTP FAMILY PROTEIN
AT4G08320

Predicted

two hybrid

two hybrid

FSW = 0.0205

Unknown

 TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN
AT4G21660

Predicted

two hybrid

two hybrid

FSW = 0.0109

Unknown

 PROLINE-RICH SPLICEOSOME-ASSOCIATED (PSP) FAMILY PROTEIN
AT5G02380

Predicted

two hybrid

FSW = 0.0552

Unknown

 MT2B (METALLOTHIONEIN 2B) COPPER ION BINDING
AT5G21274

Predicted

two hybrid

FSW = 0.0018

Unknown

 CAM6 (CALMODULIN 6) CALCIUM ION BINDING
AT5G25800

Predicted

two hybrid

two hybrid

FSW = 0.0079

Unknown

 EXONUCLEASE FAMILY PROTEIN
AT5G41770

Predicted

two hybrid

two hybrid

FSW = 0.0195

Unknown

 CROOKED NECK PROTEIN PUTATIVE / CELL CYCLE PROTEIN PUTATIVE
AT5G55760

Predicted

two hybrid

FSW = 0.0162

Unknown

 SRT1 (SIRTUIN 1) DNA BINDING / NAD OR NADH BINDING / NAD-DEPENDENT HISTONE DEACETYLASE/ ZINC ION BINDING
AT1G44820

Predicted

Affinity Capture-MS

FSW = 0.0191

Unknown

 AMINOACYLASE PUTATIVE / N-ACYL-L-AMINO-ACID AMIDOHYDROLASE PUTATIVE
AT2G22040

Predicted

Affinity Capture-MS

FSW = 0.0184

Unknown

 TRANSDUCIN FAMILY PROTEIN / WD-40 REPEAT FAMILY PROTEIN
AT2G44820

Predicted

Affinity Capture-MS

FSW = 0.0191

Unknown

 UNKNOWN PROTEIN
AT5G46150

Predicted

Affinity Capture-MS

FSW = 0.0055

Unknown

 LEM3 (LIGAND-EFFECT MODULATOR 3) FAMILY PROTEIN / CDC50 FAMILY PROTEIN
AT1G80410

Predicted

synthetic growth defect

FSW = 0.0237

Unknown

 EMB2753 (EMBRYO DEFECTIVE 2753) BINDING
AT5G13780

Predicted

synthetic growth defect

FSW = 0.0217

Unknown

 GCN5-RELATED N-ACETYLTRANSFERASE PUTATIVE
AT5G41700

Predicted

Affinity Capture-Western

FSW = 0.0076

Unknown

 UBC8 (UBIQUITIN CONJUGATING ENZYME 8) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G21000

Predicted

two hybrid

FSW = 0.0552

Unknown

 ACA6 (ALPHA CARBONIC ANHYDRASE 6) CARBONATE DEHYDRATASE/ ZINC ION BINDING
AT1G47970

Predicted

two hybrid

FSW = 0.0690

Unknown

 UNKNOWN PROTEIN

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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