Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G21250 - ( WAK1 (CELL WALL-ASSOCIATED KINASE) kinase )

3 Proteins interacs with AT1G21250
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT1G06680

Experimental

two hybrid

Reconstituted Complex

Affinity Capture-Western

biochemical

FSW = 0.0745

Class B:

vacuole

unclear

plastid

golgi

Class D:

nucleus (p = 0.78)

PSBP-1 (PHOTOSYSTEM II SUBUNIT P-1) POLY(U) BINDING
AT5G19280

Experimental

co-fractionation

Co-fractionation

FSW = 0.0832

Class B:

vacuole

unclear

plasma membrane

golgi

Class D:

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

KAPP (KINASE ASSOCIATED PROTEIN PHOSPHATASE) PHOSPHOPROTEIN PHOSPHATASE/ PROTEIN KINASE BINDING / PROTEIN SERINE/THREONINE PHOSPHATASE
AT2G05520

Experimental

pull down

molecular sieving

two hybrid

Reconstituted Complex

Affinity Capture-Western

co-fractionation

Co-fractionation

FSW = 0.1684

Class B:

vacuole

unclear

peroxisome

golgi

Class D:

nucleus (p = 0.78)

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

GRP-3 (GLYCINE-RICH PROTEIN 3)

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Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454