Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT4G10180 - ( DET1 (DE-ETIOLATED 1) catalytic )

8 Proteins interacs with AT4G10180
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT2G32950

Experimental

co-fractionation

Co-fractionation

FSW = 0.2078

Class A:

nucleus

Class B:

cytosol

 COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT5G11260

Experimental

Phenotypic Suppression

FSW = 0.1062

Class A:

nucleus

Class D:

plastid (p = 0.78)

 HY5 (ELONGATED HYPOCOTYL 5) DNA BINDING / DOUBLE-STRANDED DNA BINDING / TRANSCRIPTION FACTOR
AT4G21100

Experimental

Affinity Capture-Western

FSW = 0.3556

Class B:

nucleus

extracellular

Class D:

plastid (p = 0.78)

 DDB1B (DAMAGED DNA BINDING PROTEIN 1 B) DAMAGED DNA BINDING / PROTEIN BINDING
AT3G13550

Experimental

molecular sieving

pull down

molecular sieving

Reconstituted Complex

Affinity Capture-Western

in vitro

FSW = 0.1959

Class D:

plastid (p = 0.78)

mitochondrion (p = 0.82)

 FUS9 (FUSCA 9) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G05420

Experimental

two hybrid

Affinity Capture-Western

two hybrid

FSW = 0.3712

Class D:

plastid (p = 0.78)

 DDB1A (DAMAGED DNA BINDING PROTEIN 1A) DNA BINDING / PROTEIN BINDING
AT5G20570

Experimental

Reconstituted Complex

in vitro

FSW = 0.0834

Class D:

plastid (p = 0.78)

 RBX1 (RING-BOX 1) PROTEIN BINDING
AT5G46210

Experimental

Affinity Capture-Western

Reconstituted Complex

Affinity Capture-Western

co-fractionation

Co-fractionation

in vitro

affinity technology

FSW = 0.5193

Unknown

 CUL4 (CULLIN4) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT4G11110

Predicted

Affinity Capture-Western

FSW = 0.4931

Unknown

 SPA2 (SPA1-RELATED 2) PROTEIN BINDING / SIGNAL TRANSDUCER

Downloads

Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454

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