Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
Find locus: show subcellular location show Ontology

Export PPI as  



AtPIN will be upgraded. Please send your suggestions here

AT1G71830 - ( SERK1 (SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1) kinase/ transmembrane receptor protein serine/threonine kinase )

16 Proteins interacs with AT1G71830
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT3G09840

Experimental

two hybrid

two hybrid

fluorescence acceptor donor pair

fluorescence acceptor donor pair

protein kinase assay

protein kinase assay

confocal microscopy

pull down

fluorescence acceptor donor pair

Reconstituted Complex

biochemical

Affinity Capture-MS

Affinity Capture-Western

two hybrid

in vitro

FSW = 0.0635

Class A:

plasma membrane

endoplasmic reticulum

Class B:

unclear

nucleus

cytosol

Class D:

cytosol (p = 0.67)

CDC48 (CELL DIVISION CYCLE 48) ATPASE/ IDENTICAL PROTEIN BINDING
AT4G39400

Experimental

coimmunoprecipitation

in vitro

Affinity Capture-MS

biochemical

biochemical

fluorescence acceptor donor pair

Affinity Capture-Western

coimmunoprecipitation

affinity technology

FSW = 0.1793

Class A:

plasma membrane

Class B:

vacuole

unclear

endoplasmic reticulum

Class D:

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

BRI1 (BRASSINOSTEROID INSENSITIVE 1) KINASE/ PROTEIN BINDING / PROTEIN HETERODIMERIZATION/ PROTEIN HOMODIMERIZATION/ PROTEIN KINASE/ PROTEIN SERINE/THREONINE KINASE
AT5G10450

Experimental

two hybrid

confocal microscopy

pull down

protein kinase assay

Reconstituted Complex

biochemical

fluorescence acceptor donor pair

in vitro

FSW = 0.0270

Class A:

plasma membrane

Class B:

unclear

plastid

peroxisome

nucleus

endoplasmic reticulum

cytosol

Class D:

cytosol (p = 0.67)

GRF6 (G-BOX REGULATING FACTOR 6) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT3G02520

Experimental

coimmunoprecipitation

Affinity Capture-MS

Affinity Capture-Western

FSW = 0.0205

Class A:

plasma membrane

Class B:

unclear

plastid

endoplasmic reticulum

Class D:

cytosol (p = 0.67)

GRF7 (GENERAL REGULATORY FACTOR 7) PROTEIN BINDING / PROTEIN PHOSPHORYLATED AMINO ACID BINDING
AT4G33430

Experimental

Affinity Capture-MS

fluorescence acceptor donor pair

FSW = 0.1172

Class A:

plasma membrane

Class B:

unclear

extracellular

endoplasmic reticulum

Class D:

plasma membrane (p = 0.25)

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

BAK1 (BRI1-ASSOCIATED RECEPTOR KINASE) KINASE/ PROTEIN BINDING / PROTEIN HETERODIMERIZATION/ PROTEIN SERINE/THREONINE KINASE
AT5G19280

Experimental

two hybrid

two hybrid

Reconstituted Complex

Affinity Capture-MS

fluorescence acceptor donor pair

fluorescence acceptor donor pair

coimmunoprecipitation

two hybrid

FSW = 0.2165

Class A:

plasma membrane

Class B:

unclear

endoplasmic reticulum

Class D:

plastid (p = 0.78)

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

KAPP (KINASE ASSOCIATED PROTEIN PHOSPHATASE) PHOSPHOPROTEIN PHOSPHATASE/ PROTEIN KINASE BINDING / PROTEIN SERINE/THREONINE PHOSPHATASE
AT5G13790

Experimental

Affinity Capture-MS

FSW = 0.0401

Class A:

plasma membrane

Class B:

unclear

endoplasmic reticulum

Class D:

plastid (p = 0.78)

AGL15 (AGAMOUS-LIKE 15) DNA BINDING / TRANSCRIPTION FACTOR
AT1G34210

Experimental

fluorescence acceptor donor pair

fluorescence acceptor donor pair

FSW = 0.0936

Class A:

plasma membrane

Class B:

unclear

endoplasmic reticulum

Class D:

plasma membrane (p = 0.25)

extracellular (p = 0.86)

endoplasmic reticulum (p = 0.46)

cytosol (p = 0.67)

SERK2 (SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 2) KINASE
AT2G33500

Experimental

Affinity Capture-MS

FSW = 0.1404

Class A:

plasma membrane

Class B:

unclear

endoplasmic reticulum

ZINC FINGER (B-BOX TYPE) FAMILY PROTEIN
AT3G54840

Experimental

confocal microscopy

FSW = 0.0050

Class A:

endoplasmic reticulum

Class B:

unclear

plasma membrane

endosome

Class D:

plastid (p = 0.78)

ARA6 GTP BINDING / GTPASE
AT5G16590

Predicted

Gene fusion method

FSW = 0.5444

Class C:

plasma membrane

LRR1 ATP BINDING / KINASE/ PROTEIN SERINE/THREONINE KINASE
AT1G11050

Predicted

Gene fusion method

FSW = 0.1910

Unknown

PROTEIN KINASE FAMILY PROTEIN
AT4G28670

Predicted

Gene fusion method

FSW = 0.4545

Unknown

PROTEIN KINASE FAMILY PROTEIN
AT5G27950

Predicted

Gene fusion method

FSW = 0.3810

Unknown

KINESIN MOTOR PROTEIN-RELATED
AT2G21470

Predicted

Gene fusion method

FSW = 0.1385

Unknown

SAE2 (SUMO-ACTIVATING ENZYME 2) SUMO ACTIVATING ENZYME
AT2G36980

Predicted

Gene fusion method

FSW = 0.3403

Unknown

PENTATRICOPEPTIDE (PPR) REPEAT-CONTAINING PROTEIN

Downloads

Fasta sequences:

Proteins

DNA

Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

Learn more

How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454