Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G22770 - ( GI (GIGANTEA) )

16 Proteins interacs with AT1G22770
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT5G57360

Experimental

two hybrid

pull down

Reconstituted Complex

Affinity Capture-Western

Phenotypic Enhancement

affinity technology

in vitro

FSW = 0.1420

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

ZTL (ZEITLUPE) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT2G18915

Experimental

pull down

Reconstituted Complex

two hybrid

in vitro

FSW = 0.2094

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

LKP2 (LOV KELCH PROTEIN 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT2G32950

Experimental

Phenotypic Suppression

protein complementation assay

split-reporter assay

FSW = 0.0586

Class A:

nucleus

Class B:

cytosol

Class D:

nucleus (p = 0.78)

COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT5G15840

Experimental

Dosage Growth Defect

FSW = 0.2695

Class A:

nucleus

Class D:

nucleus (p = 0.78)

CO (CONSTANS) TRANSCRIPTION FACTOR/ TRANSCRIPTION REGULATOR/ ZINC ION BINDING
AT1G01060

Experimental

Dosage Growth Defect

Phenotypic Suppression

FSW = 0.1559

Class A:

nucleus

Class D:

nucleus (p = 0.78)

LHY (LATE ELONGATED HYPOCOTYL) DNA BINDING / TRANSCRIPTION FACTOR
AT5G61380

Experimental

Phenotypic Enhancement

FSW = 0.2475

Class A:

nucleus

Class D:

nucleus (p = 0.78)

TOC1 (TIMING OF CAB EXPRESSION 1) TRANSCRIPTION REGULATOR/ TWO-COMPONENT RESPONSE REGULATOR
AT3G11540

Experimental

two hybrid

in vitro

Reconstituted Complex

pull down

Synthetic Rescue

FSW = 0.1296

Class A:

nucleus

SPY (SPINDLY) PROTEIN N-ACETYLGLUCOSAMINYLTRANSFERASE/ PROTEIN BINDING / TRANSFERASE TRANSFERRING GLYCOSYL GROUPS
AT5G62430

Experimental

coimmunoprecipitation

pull down

Affinity Capture-Western

affinity technology

FSW = 0.1296

Class D:

nucleus (p = 0.78)

CDF1 (CYCLING DOF FACTOR 1) DNA BINDING / PROTEIN BINDING / TRANSCRIPTION FACTOR
AT1G65480

Experimental

Dosage Growth Defect

FSW = 0.1451

Class D:

nucleus (p = 0.78)

FT (FLOWERING LOCUS T) PHOSPHATIDYLETHANOLAMINE BINDING / PROTEIN BINDING
AT2G25930

Experimental

two hybrid

protein complementation assay

split-reporter assay

FSW = 0.4083

Class D:

nucleus (p = 0.78)

ELF3 (EARLY FLOWERING 3) PROTEIN C-TERMINUS BINDING / TRANSCRIPTION FACTOR
AT1G68050

Experimental

in vitro

coimmunoprecipitation

two hybrid

Reconstituted Complex

pull down

two hybrid

Affinity Capture-Western

in vitro

affinity technology

FSW = 0.1894

Unknown

FKF1 (FLAVIN-BINDING KELCH REPEAT F BOX 1) SIGNAL TRANSDUCER/ TWO-COMPONENT SENSOR/ UBIQUITIN-PROTEIN LIGASE
AT4G16360

Experimental

FSW = 0.0172

Unknown

AMP-ACTIVATED PROTEIN KINASE
AT1G27360

Experimental

Reconstituted Complex

in vitro

FSW = 0.0368

Unknown

SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 11 (SPL11)
AT2G47700

Experimental

Phenotypic Enhancement

Phenotypic Suppression

FSW = 0.3000

Unknown

ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN
AT1G77300

Experimental

Phenotypic Suppression

FSW = 0.1800

Unknown

EFS (EARLY FLOWERING IN SHORT DAYS) HISTONE METHYLTRANSFERASE(H3-K36 SPECIFIC) / HISTONE METHYLTRANSFERASE(H3-K4 SPECIFIC)
AT3G22380

Experimental

Phenotypic Enhancement

FSW = 0.3788

Unknown

TIC (TIME FOR COFFEE)

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Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454