Arabidopsis thaliana protein interaction network
Laboratório de Biologia Integrativa e Sistêmica (LaBIS)
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AT1G76030 - ( vacuolar ATP synthase subunit B / V-ATPase B subunit / vacuolar proton pump B subunit / V-ATPase 57 kDa subunit )

38 Proteins interacs with AT1G76030
LocusMethodFSW Cellular Compartment Classification (C3)Description
AT4G29130

Experimental

two hybrid

Affinity Capture-MS

Affinity Capture-Western

coimmunoprecipitation

FSW = 0.0465

Class A:

vacuole

Class B:

unclear

plastid

plasma membrane

mitochondrion

Class D:

cytosol (p = 0.67)

HXK1 (HEXOKINASE 1) ATP BINDING / FRUCTOKINASE/ GLUCOKINASE/ HEXOKINASE
AT1G50420

Experimental

two hybrid

FSW = 0.1429

Class B:

vacuole

plastid

plasma membrane

nucleus

Class D:

mitochondrion (p = 0.82)

cytosol (p = 0.67)

SCL3 TRANSCRIPTION FACTOR
AT3G11280

Experimental

two hybrid

FSW = 0.0454

Class B:

vacuole

plastid

plasma membrane

nucleus

MYB FAMILY TRANSCRIPTION FACTOR
AT5G35410

Experimental

interaction detection method

FSW = 0.0780

Unknown

SOS2 (SALT OVERLY SENSITIVE 2) KINASE/ PROTEIN KINASE
AT4G14880

Predicted

Affinity Capture-MS

FSW = 0.0276

Class C:

vacuole

plastid

plasma membrane

OASA1 (O-ACETYLSERINE (THIOL) LYASE (OAS-TL) ISOFORM A1) CYSTEINE SYNTHASE
AT4G38510

Predicted

Enriched domain pair

Phylogenetic profile method

FSW = 0.0532

Class C:

vacuole

plastid

plasma membrane

VACUOLAR ATP SYNTHASE SUBUNIT B PUTATIVE / V-ATPASE B SUBUNIT PUTATIVE / VACUOLAR PROTON PUMP B SUBUNIT PUTATIVE / V-ATPASE 57 KDA SUBUNIT PUTATIVE
AT1G78900

Predicted

Affinity Capture-MS

Affinity Capture-Western

Affinity Capture-MS

Affinity Capture-MS

co-fractionation

Co-fractionation

Affinity Capture-Western

two hybrid

co-fractionation

Co-fractionation

Affinity Capture-MS

interaction prediction

Enriched domain pair

Gene neighbors method

FSW = 0.1711

Class C:

vacuole

plastid

plasma membrane

VHA-A (VACUOLAR ATP SYNTHASE SUBUNIT A) ATP BINDING / HYDROGEN ION TRANSPORTING ATP SYNTHASE ROTATIONAL MECHANISM / HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT3G42050

Predicted

Phenotypic Suppression

Affinity Capture-MS

FSW = 0.2815

Class C:

vacuole

plastid

plasma membrane

VACUOLAR ATP SYNTHASE SUBUNIT H FAMILY PROTEIN
AT2G21410

Predicted

Affinity Capture-Western

Affinity Capture-Western

co-fractionation

Co-fractionation

Affinity Capture-MS

interaction prediction

FSW = 0.2689

Class C:

vacuole

plastid

VHA-A2 (VACUOLAR PROTON ATPASE A2) ATPASE
AT1G20260

Predicted

Enriched domain pair

Gene neighbors method

Phylogenetic profile method

FSW = 0.2404

Class C:

vacuole

plastid

HYDROGEN ION TRANSPORTING ATP SYNTHASE ROTATIONAL MECHANISM / HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT1G12840

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

FSW = 0.3227

Class C:

vacuole

plasma membrane

DET3 (DE-ETIOLATED 3) PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT3G28715

Predicted

Affinity Capture-MS

Affinity Capture-MS

interaction prediction

FSW = 0.0648

Class C:

vacuole

plasma membrane

H+-TRANSPORTING TWO-SECTOR ATPASE PUTATIVE
AT3G58730

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.2259

Class C:

vacuole

plasma membrane

VACUOLAR ATP SYNTHASE SUBUNIT D (VATD) / V-ATPASE D SUBUNIT / VACUOLAR PROTON PUMP D SUBUNIT (VATPD)
AT4G02620

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.3137

Class C:

vacuole

plasma membrane

VACUOLAR ATPASE SUBUNIT F FAMILY PROTEIN
AT2G46560

Predicted

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.1684

Class C:

vacuole

TRANSDUCIN FAMILY PROTEIN / WD-40 REPEAT FAMILY PROTEIN
AT4G23710

Predicted

interaction prediction

FSW = 0.1214

Class C:

vacuole

VAG2 HYDROLASE ACTING ON ACID ANHYDRIDES CATALYZING TRANSMEMBRANE MOVEMENT OF SUBSTANCES
AT2G30710

Predicted

Synthetic Lethality

Synthetic Lethality

interaction prediction

FSW = 0.0311

Class C:

vacuole

RABGAP/TBC DOMAIN-CONTAINING PROTEIN
AT2G28190

Predicted

Synthetic Lethality

FSW = 0.0592

Class C:

plastid

CSD2 (COPPER/ZINC SUPEROXIDE DISMUTASE 2) SUPEROXIDE DISMUTASE
AT3G12110

Predicted

Affinity Capture-Western

FSW = 0.0111

Class C:

plasma membrane

ACT11 (ACTIN-11) STRUCTURAL CONSTITUENT OF CYTOSKELETON
AT1G07670

Predicted

synthetic growth defect

FSW = 0.0414

Class C:

plasma membrane

CALCIUM-TRANSPORTING ATPASE
AT5G60790

Predicted

Affinity Capture-MS

FSW = 0.0201

Class C:

plasma membrane

ATGCN1 TRANSPORTER
AT3G10920

Predicted

Phenotypic Enhancement

FSW = 0.0142

Unknown

MSD1 (MANGANESE SUPEROXIDE DISMUTASE 1) METAL ION BINDING / SUPEROXIDE DISMUTASE
AT3G05710

Predicted

Synthetic Lethality

interaction prediction

FSW = 0.0274

Unknown

SYP43 (SYNTAXIN OF PLANTS 43) SNAP RECEPTOR
AT4G36480

Predicted

Synthetic Lethality

FSW = 0.0677

Unknown

ATLCB1 (LONG-CHAIN BASE1) PROTEIN BINDING / SERINE C-PALMITOYLTRANSFERASE
AT5G19130

Predicted

Affinity Capture-MS

FSW = 0.0493

Unknown

GPI TRANSAMIDASE COMPONENT FAMILY PROTEIN / GAA1-LIKE FAMILY PROTEIN
AT4G26840

Predicted

Affinity Capture-MS

FSW = 0.0125

Unknown

SUMO1 (SMALL UBIQUITIN-LIKE MODIFIER 1) PROTEIN BINDING / PROTEIN TAG
AT4G15900

Predicted

Affinity Capture-MS

Gene fusion method

FSW = 0.0035

Unknown

PRL1 (PLEIOTROPIC REGULATORY LOCUS 1) BASAL TRANSCRIPTION REPRESSOR/ NUCLEOTIDE BINDING / PROTEIN BINDING
AT1G30825

Predicted

Synthetic Lethality

FSW = 0.0276

Unknown

DIS2 (DISTORTED TRICHOMES 2) STRUCTURAL MOLECULE
AT1G60490

Predicted

Synthetic Lethality

FSW = 0.0296

Unknown

ATVPS34 1-PHOSPHATIDYLINOSITOL-3-KINASE/ BINDING / INOSITOL OR PHOSPHATIDYLINOSITOL KINASE/ PHOSPHOTRANSFERASE ALCOHOL GROUP AS ACCEPTOR
AT2G02760

Predicted

synthetic growth defect

FSW = 0.0270

Unknown

ATUBC2 (UBIQUITING-CONJUGATING ENZYME 2) UBIQUITIN-PROTEIN LIGASE
AT3G08560

Predicted

Co-purification

Affinity Capture-MS

FSW = 0.3137

Unknown

VHA-E2 (VACUOLAR H+-ATPASE SUBUNIT E ISOFORM 2) HYDROGEN-EXPORTING ATPASE PHOSPHORYLATIVE MECHANISM / PROTON-TRANSPORTING ATPASE ROTATIONAL MECHANISM
AT3G18430

Predicted

Synthetic Lethality

interaction prediction

FSW = 0.0990

Unknown

CALCIUM-BINDING EF HAND FAMILY PROTEIN
AT4G13720

Predicted

Affinity Capture-MS

FSW = 0.0463

Unknown

INOSINE TRIPHOSPHATE PYROPHOSPHATASE PUTATIVE / HAM1 FAMILY PROTEIN
AT4G24040

Predicted

Affinity Capture-MS

FSW = 0.0168

Unknown

TRE1 (TREHALASE 1) ALPHAALPHA-TREHALASE/ TREHALASE
AT5G12030

Predicted

Affinity Capture-MS

Affinity Capture-MS

FSW = 0.0250

Unknown

AT-HSP176A (ARABIDOPSIS THALIANA HEAT SHOCK PROTEIN 176A) UNFOLDED PROTEIN BINDING
AT5G42190

Predicted

Affinity Capture-Western

Affinity Capture-MS

interaction prediction

FSW = 0.0227

Unknown

ASK2 (ARABIDOPSIS SKP1-LIKE 2) PROTEIN BINDING / UBIQUITIN-PROTEIN LIGASE
AT5G65180

Predicted

Affinity Capture-MS

FSW = 0.0175

Unknown

FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN EXPRESSED IN 18 PLANT STRUCTURES EXPRESSED DURING 9 GROWTH STAGES CONTAINS INTERPRO DOMAIN/S PROTEIN OF UNKNOWN FUNCTION DUF618 (INTERPROIPR006903) REGULATION OF NUCLEAR PRE-MRNA PROTEIN (INTERPROIPR006569) ENTH/VHS (INTERPROIPR008942) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UNKNOWN PROTEIN (TAIRAT5G100601) HAS 3523 BLAST HITS TO 3241 PROTEINS IN 333 SPECIES ARCHAE - 25 BACTERIA - 251 METAZOA - 1575 FUNGI - 421 PLANTS - 142 VIRUSES - 17 OTHER EUKARYOTES - 1092 (SOURCE NCBI BLINK)
AT2G27600

Predicted

Synthetic Lethality

FSW = 0.0186

Unknown

SKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) ATP BINDING / NUCLEOSIDE-TRIPHOSPHATASE/ NUCLEOTIDE BINDING

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Fasta sequences:

Proteins

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Quick help

Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated from
Predicted: The indicated PPI was proposed based on ortholgs studies. The experiment indicated links to the publication of the orthologous PPI was annotated from

FSW

FSW is the Functional Similarity Weight. It represents the proportion of interaction partners that two proteins have in common

Learn more - FSWeight top ranked cut offs

C3

Class A: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated and both, target and source, were indicated as expressed in same cellular compartment
Class B: The PPI is a direct evidence (experimental), the subcellular location was experimentally demonstrated but both, target and source, were indicated as expressed in different cellular compartment
Class C: Same as Class A but the PPI is predicted
Class D: Same as Class A but subcellular location was predicted. P(exp|pred) indicates the probability of this particular predicted location be experimentally demonstrated given all data available on AtPINDB
Unknown: There is no available data to calculate the C3 or the data does not fit onto any class previously described

PEP

PEP is the posterior probability of this particular PPI be experimentally demonstrated once it was predicted. For this release p = 0.0030. If they share same cell compartment p = 0.0029

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How to cite

If you find AtPIN interesting and want to use it in your work please cite us

AtPIN: Arabidopsis thaliana Protein Interaction Network
Marcelo M Brandao, Luiza L Dantas and Marcio C Silva-Filho
BMC Bioinformatics 2009, 10:454

DOI:1471-2105/10/454